Notch信号在急性早幼粒细胞白血病患者骨髓中的表达
Notch signal expression in the bone marrow of patients with acute promyelocytic leukemia
张丽 1夏德雨 1李国辉 1蒋姗姗 1谷芳娜 1梁英民1
作者信息
- 1. 第网军医大学唐都医院血液科,陕西,西安,710038
- 折叠
摘要
目的:通过检测急性早幼粒细胞性白血病(APL)患者治疗前后骨髓中Notch信号途径中受体、配体和靶基因的表达情况,探讨其在APL发生中的作用.方法:采用半定量RT-PCR技术检测APL患者治疗前后骨髓中Noteh1,Noteh2,Noteh3,Noteh4,Delta-like-1,Delta-like-4,Jagged1,Jagged2,Hes1,Hes5的表达.结果:受体Notch2、配体Jagged1、靶基因Hes1的表达在APL完全缓解后明显下调,其它受体、配体及靶基凶的变化尤统计学意义.结论:在APL患者治疗前后存在Notch基凶的改变,Notch信号的这些改变在APL的发病机制中可能有重要作用.
Abstract
AIM:To explore the possible role of Notch signaling in occurrence of acute promyelocytic leukemia(APL)through investigate the expression of Notch receptors, ligands and target gene in the bone marrow of patients with APL.METHODS:Semi-quantitative reverse transeriptase-polymerase chain reaction(RT-PCR)was used to investigate the expression of Notch1,Notch2,Notch3,Notch4,Delta-like-1,Delta-like-4,Jagged1,Hes1, Hes5 in the bone marrow of patients with APL,and the changes of expression after treatment.RESULTS:The expres-sions of Notch2, Jaggedl and Hesl were significantly lower after treatment, the expressions of the others have no significant difference. CONCLUSION:The changes of Notch2, Jagged1 and Hes1 expression after treatment are significant. The difference on the expression of Notch receptors and ligands may be important significance for the pathogenesis of APL.
关键词
Notch/急性早幼粒细胞白血病Key words
Notch/acute promyelocytic leukemia引用本文复制引用
基金项目
国家高技术研究发展计划(863计划)(2006AA02A111)
出版年
2009
NETL
NSTL
维普
万方数据