DACH1蛋白在甲状腺乳头状癌中的表达及其意义
Immunohistochemic expression of DACH1 and its clinical significance in papillary thyroid carcinoma
赵昕 1周洁 1司翠 1王文勇 2张南雁 1王晨光 3姬秋和1
作者信息
- 1. 第四军医大学西京医院内分泌代谢科,陕西,西安,710033
- 2. 第四军医大学基础部病理学教研室,陕西,西安,710033
- 3. 美国托马斯杰佛逊大学
- 折叠
摘要
目的:探讨DACH1的表达与甲状腺乳头状癌的发生、发展及预后的关系.方法:收集临床病理标本40例,其中甲状腺乳头状癌30例、结节性甲状腺肿10例.运用免疫组织化学SABC法,以多克隆抗体兔抗人DACH1进行免疫组织化学染色,镜下观察不同甲状腺组织巾DACH1的表达,比较其阳件率,分析其与临床病理特件的相关性.结果:DACH1蛋白在甲状腺乳头状癌中表达阳性率高达76.7%(23/30),其中强阳性药46.6%(14/30).而DACH1在结节性甲状腺肿中的表达仅为30%(3/10),且仅为弱阳性.统计分析表明具有显著性差异(P<0.01).而且DACHI蛋白的表达与甲状腺乳头状痛有否淋巴结转移、有无包膜外侵犯有关(P<0.05),而与年龄、肿瘤大小、临床分期无关(P>0.05).结论:DACH1的异常表达可能参与甲状腺乳头状癌的发生发展,在其发病中起重要作用.
Abstract
AIM:To explore the immunohistochemical expression of DACH1 in papillary thyroid carcinoma and its clinical significance. METHODS:DACH1 expression in 10 nodular goiters, and 30 papillary thyroid carcinomas were detected with SABC immunohistochemical method, and the relationship between DACH1 expression and clinicopathological characteristics were further analyzed. RESULTS:The immunohistochemic expression of DACHl in papillary thyroid carcinoma and goiter is orientated as the cytomembrane and cytoplasm. The positive rate of DACH1 is 76.7% in papillary thyroid carcinoma, notably higher than that in nodular goiter, later is 30%(P<0.01). The positive rate of expression of DACH1 protein in papillary thyroid carcinoma with lymph nodes metastasis was 89. 5%, while it was 54. 5% in group of without lymph nodes metastasis. The differences are significant(P<0.05). CONCLUSION:The DACH1 protein is immunohistochemic overpressed in papillary thyroid carcinoma.There is obvious relationship between the degree of infiltration and metastasis of lymph node, indicating that DACHI may play a role in the carcinogenesis and development of papillary thyroid carcinoma.
关键词
DACH1蛋白/甲状腺乳头状癌/免疫组织化学Key words
DACH1/papillary thyroid carcinoma/immunohistochemistry引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据