维氏气单胞菌拮抗菌株J2-2的筛选、鉴定与效果评价
Isolation,identification and effect evaluation of antagonistic strain J2-2 from Aeromonas veronii
程心怡 1孙正祥 1苏应兵 2周燚1
作者信息
- 1. 长江大学农学院, 湖北荆州 434025
- 2. 长江大学动物科学学院, 湖北荆州 434025
- 折叠
摘要
为筛选对维氏气单胞菌(Aeromonas veronii)有潜在抑菌作用的菌株,本研究从湖北公安县泥鳅养殖池塘底泥中分离一株细菌J2-2,对其特性进行了研究,并对抑菌活性物质进行了初步分析.结果显示:菌株J2-2 为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),具有抑菌广谱性,对米尔伊丽莎白菌(Elizabethella milieri)、嗜水气单胞菌(A.hydrophila)等6 种水产病原菌的抑菌直径在9.6～29.5 mm.菌株J2-2 的抑菌物质具有较好的酸碱稳定性、热稳定性和蛋白酶K处理的稳定性.电镜结果显示菌株J2-2 的发酵产物对维氏气单胞菌的细胞膜结构具有破坏作用.通过LC-MC分析该拮抗细菌可产生至少 15 种不同的脂肽类化合物如杆菌霉素等.药敏试验结果显示,菌株J2-2 对氨苄西林、青霉素具有耐药性,对克林霉素中度敏感,对大多数抗生素高度敏感.
Abstract
This study isolated a strain J2-2 from the bottom mud of loach aquaculture pond in Gongan County,Hubei Province,to screen the bacteria with potential antibacterial effect on Aeromonas veronii.Meanwhile,its char-acteristics were studied and the antibacterial active substances were preliminarily analyzed.The results showed that the strain J2-2 is Bacillus amyloliquefaciens,which has a broad bacteriostatic spectrum.The inhibition diameters of 6 aquatic pathogens,such as Elizabethella milieri and A.hydrophila,ranged from 9.6 to 29.5mm.The bacterio-static substances of strain J2-2 were insensitive to high temperature,acid,acid-base,and protease.The results of electron microscopy showed that the fermentation product of strain J2-2 had destructive effect on the cell mem-brane structure of A.veronii.Through LC-MC analysis,the antagonistic bacteria could produce at least 15 li-popeptide compounds such as Bacillomycin D,etc.The susceptibility test results showed that strain J2-2 was on-ly resistant to ampicillin and penicillin,moderately susceptible to clindamycin and highly susceptible to most anti-biotics.
关键词
解淀粉芽孢杆菌(Bacillus/amyloliquefaciens)/维氏气单胞菌(Aeromonas/veronii)/安全性评价/LC-MSKey words
Bacillus amyloliquefaciens/Aeromonas veronii/safety evaluation/LC-MS引用本文复制引用
基金项目
湖北省重点研发计划(2020BBA051)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据