摘要
为研究恩诺沙星及其代谢产物环丙沙星在牛蛙(Lithobates catesbeiana)体内药代动力学及分布,明确恩诺沙星在牛蛙体内的最大残留限量及休药期.本实验进行牛蛙单次口灌 10%恩诺沙星粉混悬液(20 mg/kg·bw),在0.5、1、2、4、8、12、24、48、96、192、336、480 h分组织取样检测实验.研究显示:恩诺沙星和环丙沙星在牛蛙血液中的达峰时间(Tmax)相同为1h,峰浓度(Cmax)分别为 26.592 μg/mL、2.256 μg/mL;恩诺沙星在牛蛙肌肉、肝脏、肾脏、脑中的Tmax均为8h,Cmax分别为10.21、13.84、17.52、3.95 mg/kg;环丙沙星在牛蛙肌肉、肝脏、肾脏的Tmax分别为12、8、12 h,Cmax分别为1.362、20.46、4.07 mg/kg,在脑中未检测出环丙沙星.结果表明:恩诺沙星在牛蛙体内吸收迅速,21.90%～40.35%恩诺沙星转化为环丙沙星,与鱼类和甲壳类生物相比,环丙沙星在牛蛙体内更易消除;恩诺沙星在牛蛙体内的最大残留限量MRL为108.22 μg/kg;单剂量10%恩诺沙星粉(20 mg/kg bw)适用于治疗由于嗜水气单胞菌(MIC:0.1 μg/mL)患病牛蛙;牛蛙在室温为25℃左右的饲养环境下,建议休药期不低于514.24 h(22 d).
Abstract
The purpose of this experiment is to study the pharmacokinetics and distribution of Enrofloxacin and its metabolite Ciprofloxacin in Lithobates catesbeiana,and to determine the Maximum Residue Limit of Enrofloxa-cin in L.catesbeiana and its drug withdrawal period.L.catesbeiana were given a single oral infusion of 10%Enro-floxacin powder suspension(20 mg/kg·bw),and samples were taken at 0.5,1,2,4,8,12,24,48,96,192,336 and 480 h.The Tmax of Enrofloxacin and Ciprofloxacin in blood was the same as 1 h,and the Cmax was 26.59,2.26 mg/kg,respectively;The Tmax of Enrofloxacin in muscle,liver,kidney and brain was the same as 8 h,and the Cmax was 10.21,13.84,17.52 and 3.95 mg/kg,respectively.The Tmax of Cipro-floxacin in muscle,liver,and kidney were 12,8,and 12 h,respectively,and the Cmax was 1.36,20.46 and 4.07 mg/kg,respectively.No Ciprofloxacin was detected in the brain.The results showed that Enrofloxa-cin was rapidly absorbed in L.catesbeiana,with 21.90%～40.35%being converted into Ciprofloxacin,and compared to fish and crustaceans,Ciprofloxacin is more easily eliminated in L.catesbeiana;The MRL of Enro-floxacin in L.catesbeiana is 108.22 μg/kg;A single dose of 10%Enrofloxacin powder(20 mg/kg bw)is suitable for treating of L.catesbeiana infected with Aeromonas hydrophila(MIC:0.1 μg/mL).L.catesbeiana are recommended to rest for no less than 514.24 hours(22 days)at a room temperature of around 25℃.
基金项目
农产品质量安全风险评估项目(GJFP20230402)
国家淡水水产种质资源库建设项目(FGRC:18537)
国家科技期刊平台
NSTL
维普
万方数据