A total of 480 juvenile Ctenopharyngodon idella with an initial body weight of(4.29±0.18) g were ran-domly allocated into four groups(four replicates per group;30 fish per replicate) and fed with four diets, re-spectively to assess the impact of zinc sulfate, zinc methionine, and their combination on growth, body com-position, zinc accumulation, serum biochemistry, and antioxidation in C.idella.The control diet(D1 group) contained no additional zinc supplementation.The other three diets contained 60 mg/kg of different forms of zinc compared to the control group: D2-zinc sulfate, D3-zinc methionine, and D4-a mixture containing 30 mg/kg each from the abovementioned sources.The results demonstrated that the growth performance and total zinc content in the D2-D4 group were significantly higher than those in the D1 group, and the crude fat con-tent in the D2-D4 group was significantly lower than that in the D1 group.There was no significant difference in growth performance,total body composition, and zinc content between the D2-D4 groups.Group D4 dis-played the highest serum total protein content and aspartate aminotransferase activity, followed by groups D2 and D3, with group D1 exhibiting the lowest levels observed.Group D1 also presented the highest alanine ami-notransferase activity among all analyzed groups.The CAT activity of the D1 group was significantly lower, while the MDA content was significantly higher than the other three groups.Hepatocytes in the D1 group exhib-ited vacuolation, blurred membrane boundaries, and nuclear migration, whereas zinc supplementation resul-ted in macroscopic improvements in liver morphology.In conclusion,our findings demonstrate that dietary sup-plementation with 60 mg/kg of zinc sulfate, zinc methionine, or mixed zinc can enhance antioxidant capacity and promote growth performance and health status in C.idella.Notably,mixed sources of zinc appear to confer superior health benefits on this species.
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