The differences of Cipangopaludina chinensis gut microorganisms at different developmental stages in the same habitat and the comparative research with sediment microorganisms were studied to provide a reference for the construction of an ecological integrated rice-snail breeding model.In this study,the 16S rRNA amplicons were sequenced using the Ion S5TMXL platform to comparatively study the adult and juvenile snail gut flora and their functions.We found that the microbial diversity in the sediment was significantly higher than that of the snail gut flora(P<0.05).In comparison,the diversity of the juvenile snail gut flora was also significantly higher than that of the adult snail(P<0.05).A total of 51 phyla and 243 genera were identified in all samples, in which Bacteroidetes(48.95%), Proteobacteria(20.37%) and Firmicutes(7.94%) dominated.Indicator spe-cies analysis revealed that the abundance of five bacterial groups(Bryobacter,Syntrophus, Acidibacter, Nitrospira and Roseomonas) in the gut of juvenile snails, all associated with the metabolism of energy substances such as pro-teins and amino acids, were significantly higher than that of adult snails(P<0.05), implying that more energy substances were required and more digestive and metabolic capacity in the juvenile period.The results of func-tional prediction showed that the abundance of functional flora of sugar metabolism,amino acid metabolism, energy metabolism, cofactor and vitamin metabolism in the snail gut was significantly higher than that in the sediment(P<0.01).Still,there was no difference in the function of the gut flora of adult and juvenile snails(P>0.05), indicating that there was a specific preference for the tasks performed by the flora in different habitats of the gut and the sediment.In contrast,the function of the gut flora of snails in the same environment was more consistent.The microbial abundance of snail gut and sediment are different.Still,the microbial community com-position of the two is similar, indicating that the changes in the microbial composition of snail gut are closely re-lated to the substrate in the same ecosystem.Meanwhile,the composition of gut flora and its function are more consistent during the growth of snails.Still,the juvenile snails in the rapid growth stage have a metabolic solid capacity for energy substances, so this stage should be given sufficient nutrition.The proportion of fat,protein and other energy substances in the feed should be appropriately increased to meet the energy demand of the rap-id growth of snails.
维普
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