The present study was carried out by a previous In this study, species identification, inter-popula-tion genetic distance and variation to investigate the population genetic structure of five indigenous fish species of Schizothorax biddulphi, S.labrosus, Diptychus maculatus, Triplophysa tenuis and T.stoliczkae parasitizing Contracaecum sp.within the Yarkand River Basin.Meanwhile, population structure were analyzed by the ribosomal ITS-1 gene and mitochondrial COⅡ gene of a total of 112 Contracaecum sp.hosts.The comparison results of the evolu-tionary tree constructed by the neighbour-joining method showed thatall the five host fish species of Contracaecum sp.were Contracaecum rudolphii B based on the homology analysis of the ribosomal ITS-1 gene in NCBI website.A total of 71 haplotypes were detected based on the COⅡ gene; one shared haplotype was Hap6, and the other was Hap30, which was only Schizothoracine.Hap30 occurred only in the Schizothorax population.The diversi-ty analysis showed that the haplotype diversity(hd) of the five populations ranged from 0.714 to 0.978.The nucleotide diversity(Pi) ranged from 0.00540 to 0.01091.Among them, the Hd and Pi of the T.stoliczkae population were the lowest, with the values of 0.714 and 0.00540; and those of the D.maculatus population were the highest, with the values of 0.978 and 0.01091.The neighboring population had the highest values, with values of 0.978 and 0.978, while the neighboring population had the highest values.Neighbor-joining methods of constructing evolutionary trees and haplotype network diagrams showed no lineage corresponding to the host was formed among the five populations.The genetic differentiation indices ranged from -0.0013 to 0.1086.Population genetic structure and AMOVA analyses showed that the five populations were weakly dif-ferentiated and had small genetic distances, with inter-population and intra-population variation accounting for 4.33% and 95.67%, respectively.The five populations mainly derived their genetic variation within the popu-lations.It was concluded that the five populations of C.rudolphii B parasitized in the Yarkant River Basin were highly genetically diverse, with low differentiation, no host specificity, and frequent inter-population gene ex-changes.In summary, the study of the population genetic structure and differentiation information of Contracae-cum sp.aims to provide primary data for its molecular epidemiology and the conservation of indigenous fish germplasm resources in the Yarkand River Basin.
维普
万方数据
