The effect of fish meal replacement by earthworm by-products on the growth and muscle quality of largemouth bass(Micropterus Salmoides)
The effects of replacing fishmeal with earthworm by-products on the growth performance,conven-tional nutrients,water holding properties,muscle texture characteristics and muscle fiber growth of largemouth bass(Micropterus salmoides)was investigated to evaluate the appropriate proportion of earthworm by-products to replace fishmeal,alleviate the restriction of fishmeal shortage on the development of healthy aquaculture produc-tion,and realize the recycling of resources by turning waste into treasure.Earthworm by-product with different substitution ratios(0％,10％,20％,30％)was selected to replace fishmeal,which was recorded as Control,D10,D20 and D30 groups,respectively,and was cultured for four months.The results showed that compared with the Control group,the average weight and specific growth rate of largemouth bass in the D10 group were significantly increased.The muscle water holding capacity of the D20 group was significantly improved.The muscle elasticity and recovery were significantly reduced.With the increase of the substitution ratio of earth-worm by-products,the percentage of protein content and muscle fiber area(1000-5000 μm2)in whole fish and muscle of largemouth bass increased significantly.There was no significant difference in the enzyme activi-ties of AKP,GOT and GPT.Earthworm by-products could promote myofiber proliferation and growth by im-proving growth performance and up-regulating the expression levels of muscle growth-related genes(scopole-tin,myogenic regulatory factor 6,insulin-like growth factor 1 and insulin-like growth factor 2),thereby impro-ving muscle quality.The D20 group was significantly higher than the Control group,which included the muscle water-holding capacity,protein content,muscle fiber area(1000-5000 μm2)and the expression level of insu-lin-like growth factor 1 genes.The muscle springiness and resilience of the D20 group were significantly lower than that of the Control group.Therefore,based on a comprehensive analysis of growth performance and mus-cle quality data,it is recommended that the optimal replacement ratio of earthworm by-products to fish meal in the diet of largemouth bass is 20％.
万方数据
维普
