首页|克氏原螯虾碳、氮稳定同位素周转与分馏特征研究

克氏原螯虾碳、氮稳定同位素周转与分馏特征研究

扫码查看
原文链接
  • 万方数据
  • 维普
为了解稳定同位素在克氏原螯虾(Procambarus clarkii)体内的周转与分馏特征,通过室内控制实验,研究了两种不同规格(虾种、成虾)克氏原螯虾在不同投饲条件下(饲料、鱼糜)的碳、氮稳定同位素周转半衰期和肌肉组织判别系数.结果表明,饲料组和鱼糜组虾种的生长系数分别为(0.008 5±0.000 7)d-1、(0.007 9±0.001 2)d-1,成虾的生长系数分别为(0.001 3±0.000 1)d-1、(0.000 9±0.000 4)d-1,不同投饲条件下克氏原螯虾生长系数差异不显著.虾种的相对增长率(1.60、1.57)大于成虾(1.08、1.05),不同投饲条件下克氏原螯虾相对增长率差异不大.相对于饲料组,鱼糜组虾种碳稳定同位素比值(δ13C)在第7天出现显著差异,在28d至56 d趋于稳定;成虾组δ13C值在第14 d出现显著差异,在28 d至56 d趋于稳定;虾种和成虾氮稳定同位素比值(δ15N)均在第14 d出现显著差异,在28 d至56 d趋于稳定.虾种碳稳定同位素周转速率(0.020 8±0.004 0)d-1大于成虾(0.018 4±0.002 0)d-1.虾种和成虾的碳同位素判别系数分别为0.89‰±0.15‰、1.06‰±0.16‰,氮同位素判别系数分别为3.42‰±0.08‰、3.63‰±0.13‰,虾种的碳同位素判别系数、氮同位素判别系数均小于成虾.研究结果可为应用碳、氮稳定同位素技术开展克氏原螯虾食性研究提供基础参数,提高食源分析的准确性.
Studies on the turnover and fractionation characteristics of stable isotopes of carbon and nitrogen in Procambarus clarkii
To understand the turnover and fractionation characteristics of carbon and nitrogen stable isotopes in Procambarus clarkii,the turnover half-life of carbon and nitrogen stable isotopes and the muscle tissue discrimina-tion factor of two different sizes of P.clarkii(juvenile and adult)under different feeding conditions(feed and fish mince)were investigated in an indoor controlled experiment.The results showed that there were no significant differences in the growth coefficients between P.clarkii(juvenile)fed with feed(0.008 5±0.000 7)d-1 and those fed with fish mince(0.007 9±0.001 2)d-1.The differences in the growth coefficients between P.clarkii(adult)fed with feed(0.001 3±0.000 1)d-1 and those fed with fish mince(0.000 9±0.000 4)d-1 were no significant too.The relative growth rate of P.clarkii(juvenile)group(1.60,1.57)was higher than that of P.clarkii(adult)(1.08,1.05),while the relative growth rates between the feed and fish mince groups showed no significant differences.Compared to the feed group,the fish mince group exhibited a significant difference in the carbon sta-ble isotope ratio(δ13C)of the original crayfish species on the 7 d,which stabilized from 28 to 56 d.The carbon stable isotope ratio of P.clarkii(adult)group showed significant differences on the 14 d,also stabilizing from 28 to 56 d.Moreover,the nitrogen stable isotope ratios(815N)of both P.clarkii(juvenile and adult)displayed sig-nificant differences on the 14th day,subsequently stabilizing from 28 to 56 d.The turnover rate of stable carbon isotopes in P.clarkii(juvenile)(0.0208±0.004 0)d-1 was higher than that in P.clarkii(adult)(0.018 4±0.002 0)d-1.The carbon stable isotope discrimination factor of P.clarkii(juvenile and adult)were 0.89‰±0.15‰ and 1.06‰±0.16‰,respectively.Similarly,the nitrogen stable isotope discrimination factor of P.clarkii(juvenile and adult)were 3.42‰±0.08‰and 3.63‰±0.13‰,respectively.It is worth noting that the carbon and nitrogen stable isotope discrimination factor of P.clarkii(juvenile)were smaller than P.clarkii(adult).These results provide fundamental parameters for the application of carbon and nitrogen stable isotope techniques,thereby enhancing the accuracy of food source analysis in P.clarkii.

Procambarus clarkiicarbon turnover ratediscrimination factorcarbon stable isotope rationitrogen stable isotope ratio

陈晓龙、胡乐、熊飞、喻记新、翟东东、刘红艳、陈元元

展开 >

江汉大学生命科学学院,湖北省汉江流域特色生物资源保护开发与利用工程技术研究中心,武汉 430056

江汉大学持久性有毒污染物环境与健康危害湖北省重点实验室,武汉 430056

克氏原螯虾(Procambarus clarkii) 碳周转速率 稳定同位素判别系数 碳稳定同位素比值 氮稳定同位素比值

国家自然科学基金江汉大学一流学科建设重大专项资助计划湖北省高等学校优秀中青年科技创新团队计划项目

32102104592023XKZ025T2020034

2024

淡水渔业
中国水产学会 中国水产科学研究院长江水产研究所 中国水产科学研究院淡水渔业研究中心

淡水渔业

CSTPCD北大核心
影响因子：0.776
ISSN：1000-6907
年,卷(期)：2024.54(5)