Effects ofalkalinity on tolerance and the antioxidant capacity of juvenile crab Eniocheir sinensis
The area of saline-alkali water in China is about 46 000 million hectares.The cultivation in saline-alkali water is one of the ten models of comprehensive utilization of saline-alkali resources.The development of saline-alkaline aquaculture can effectively utilize resources and improve economic and ecological benefits.Ac-cording to the types and characteristics of saline-alkaline water resources,most of the inland areas in China are carbonate-type waters,especially in Inner Mongolia,Ningxia,Xinjiang,etc.,it is of great significance to ex-plore the best economic varieties suitable for local breeding.Since Eriocheir sinensis is highly adaptable to the envi-ronment,can live in saline-alkaline water,and has high economic value.It is essential to study the adaptability of E.sinensis to the alkalinity of carbonate.Alkalinity is an important water quality indicator that affects the growth of E.sinensis,especially for the breeding and promotion of new varieties in saline alkali water areas,which have great significance.The effects of various levels of alkalinity stress(7,9.49,12.86,17.42,23.61 and 32.00 mmol/L)in E.sinensis were investigated utilizing measuring the survival rate and antioxidant enzyme activity of juvenile crabs on the new variety of E.sinensis,"Jianghai 21"at 0,24,48 and 96 h.The results showed that the mortality rate increased as the alkalinity of Chinese mitten crab larvae increased.The median le-thal concentration(LC 50)for alkalinity at 24,48,and 96 hours were 62.32,46.77,and 34.22 mmol/L,respectively,and the safe concentration was 7.90 mmol/L.There was no significant difference in the superox-ide dismutase(SOD)activity of the control group at 7 mmol/L.In contrast,the experimental groups at 9.49,12.86,17.42,and 23.61 mmol/L showed a significant increase followed by a decrease,reaching its peak at 24 hours,while the 32 mmol/L showed a significant increase.The activities of catalase(CAT)in each experi-mental group were significantly lower than that of the control group,and the CAT activity of the alkalinity groups at concentrations of 9.49,12.86,and 17.42 mmol/L showed a decreasing trend with the change of stress time.The high-concentration alkalinity groups of 23.61 and 32 mmol/L showed an overall trend of first decreasing and then increasing.The total antioxidant capacity(T-AOC)activities in each concentration group generally showed a trend of a first increasing and then decreasing during the 96-h stress process,while no significant changes were observed in the control group during this period.The activities of glutathione peroxi-dase(GSH-Px)in the 32 mmol/L concentration group showed a significant increase trend.In contrast,other ex-perimental groups showed a trend of first significantly increasing and then decreasing,reaching a peak at 24 hours.Different alkalinity has a significant impact on the survival of E.sinensis.E.sinensis can adapt to a certain degree of alkalinity stress by activating its antioxidant system.
Eniocheir sinensisjuvenile Chinese mitten crabalkalinityantioxidant enzyme
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