Effects of incubation temperature on sex and quality of Chinemys reevesii
This experiment incubated Chinemys reevesii eggs at five constant temperatures(25,27,29,31,33 ℃)and one shifting-temperature(33 ℃ shift to 29 ℃)to improve incubation conditions and assess the effect of differing temperatures on the gender and quality.We then measured the hatching rate,morphological traits,sports performance,and physiological and biochemical indexes of C.reevesii.The findings demonstrated that incubation at 33 ℃ resulted in the shortest incubation period and the highest female ratio compared to 25 ℃,27 ℃ and 29 ℃.There was no significant difference in the percentage of females incubated at 33 ℃ and those incubated at the rotating temperature.The hatching rate was the highest at 27 ℃(42.67%),and the lar-gest measurements for tail length,carapace length,carapace width,height and plastron length were also ob-served at shifting temperature.The C.reevesii hatched at 29 ℃ exhibited the fastest swimming speed,significant-ly different from those hatched at 25 ℃ and 33 ℃.The C.reevesii hatched at 27 ℃ exhibited the fastest righting speed,significantly different from those hatched at 31 ℃ and 33 ℃.Among the physiological and biochemical indices of C.reevesii,only the total antioxidant capacity showed a significant difference,with the lowest concen-tration observed at 33 ℃.The shifting-temperature group was between 29 ℃ and 33 ℃,showing no signifi-cant difference.In conclusion,high-quality C.reeveisi can be produced by treatment at 27 ℃,29 ℃,and shift-ing temperature.Compared to constant temperatures of 27 ℃ and 29 ℃,incubation at shifting temperature re-sulted in a shorter incubation period and a higher female ratio.Additionally,the incubation quality at shifting temperature was better than incubation at 31 ℃ and 33 ℃.Therefore,the shifting-temperature method de-scribed in this article can be used as a production method for large-scale propagation of all female turtle seed-lings.