A total of 480 Micropterus salmoides with an initial weight of(3.31±0.01)g were randomly selected and divided into four groups,with four replicates in each group and 30 fish in each replicate to study the effects of adding mulberry branch biochar to feed on the growth performance,body composition,serum biochemistry and antioxidant indicators of M.salmoides.During the 42-day feeding period,experimental feeds supplemented with 0,10,20 and 40 g/kg of mulberry branch biochar were fed respectively.After the breeding,each group′s total weight and tail number were counted,growth performance such as survival rate,weight gain rate,feed coefficient and specific growth rate were calculated,and serum biochemical indicators and serum and liver an-tioxidant indicators were detected.The results showed that adding mulberry branch biochar to the feed had no significant effect on the growth performance of M.salmoides,the crude fat content showed a significant downward trend with the increase in the amount of mulberry biochar added.The serum urea,triglyceride,cholesterol and aspartate aminotransferase contents of M.salmoides in the mulberry branch biochar group were significantly re-duced.The serum total antioxidant capacity(T-AOC)of M.salmoides showed a trend of first increasing and then decreasing.The serum malondialdehyde(MDA)content decreased significantly with adding mulberry biochar.The liver T-AOC and SOD activities of each mulberry branch biochar-supplemented group were significantly higher than those of the control group.The liver MDA content of the mulberry branch biochar supplemented group was significantly reduced.The liver MDA content of the 20 g/kg group was the lowest.The serum and liver glutathione peroxidase(GSH-Px)activities in the 20 g/kg group were significantly increased.Based on the comprehensive evaluation of growth performance and antioxidant indicators,the addition level of 10 to 20 g/kg in M.salmoides feed has better effects.
维普
万方数据
