Genetic diversity and genetic structure of seven farming populations of Procambarus clarkii from Ezhou(EZ),Gongan(GA),Honghu(HH),Jianli(JLi),Jiangling(JL),Qianjiang(QJ)and Shishou(SS)were studied using ten microsatellite markers to understand the genetic background of P.clarkii populations in the main farming areas in Hubei province.Analysis of molecular variationce(AMOVA)revealed that about 6.42%of genetic variation was derived from inter-populations,and 93.58%of genetic variation from intra-popula-tions.The genetic differentiation coefficient(Fst)indicated no high genetic differentiation among the popula-tions,and there was the most extensive gene flow between Honghu and Jiangling.The UPGMA phylogenetic tree constructed based on the Nei's distance shows that the seven populations are divided into three groups,Ezhou,Gongan and Qianjiang populations as one group,Honghu,Jianli and Jiangling populations as anoth-er group,and the Shishou population as its group,respectively.The analysis revealed that the seven crayfish populations are most likely divided into five sub-populations.The seven Procambarus clarkii populations in this study have moderate genetic diversity,with the Ezhou population having the highest genetic diversity and poten-tial for selection and breeding.
Procambarus clarkiiaquaculture populations in Hubeimicrosatellitegenetic diversitygenetic struc-ture
维普
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