Effect of MUC2 Gene Silencing on Tight Junction Protein Expression in Caco-2/HT-29 Co-culture Cells by Lipopolysaccharides Interference
The first line of defense against the invasion of pathogenic substances by the intestinal mucosal barrier is the intestinal mucus layer composed of mucin-2(MUC2),which covers the surface of epithelial cells and is secreted by goblet cells.Lipopolysaccharides(LPS)can interfere with the normal functioning of the intestinal barrier by damaging the intestinal mucosa.Based on this,this study used Caco-2/HT-29(3∶1)co culture to simulate intestinal epithelial cells,and analyzed the effect of LPS on the intestinal mucosal barrier and its tight junction structure by silencing MUC2(siMUC2).The results showed that compared with the control group,the expression levels of E-cadherin and occlusins mRNA in the LPS group were sig-nificantly reduced.Compared with the LPS group,the levels of claudin,JAMA,E-cadherin,and desmosome mRNA in the LPS+siMUC2 group were significantly reduced.Downregulation of cell junction protein mR-NA levels may affect cell permeability and lead to barrier dysfunction.In summary,MUC2,as a component of the mucus layer skeleton protein,can protect cell tight junction proteins from LPS interference.LPS can induce an increase in MUC2 expression and a downregulation of E-cadherin and occludins mRNA expres-sion in Caco-2/HT-29 cell models.When the MUC2 gene is silenced,LPS can lead to a significant decrease in the expression of tight junction protein mRNA,indicating that the barrier function of tight junctions in intestinal epithelial cells may be disrupted.
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