Roles of Osteopontin and p38MAPK Signaling Pathway in Development of Echinococcus multilocularis Protoscoleces
To clarify the role of osteopontin(OPN)and Echinococcus multilocularis(Em)p38 mitogen-ac-tivated protein kinase(p38MAPK)signaling pathways in Em development,long-clauded gerbils infected with Em for 4-6 months were dissected.After extracting the Em protoscoleces,the Em protoscoleces were treated with different concentrations of a p38MAPK inhibitor(SB202190).After selecting the appropriate concentration of SB202190,the protoscoleces were divided into a DMSO group,an anti-p38MAPK group,a PBS group,an OPN group,and an OPN+anti-p38MAPK group in vitro.Eosin staining and the caspase-3 kit were used to detect caspase-3;EDU and Hoechst staining and the reactive oxygen species(ROS)probe were used to detect the activity,apoptosis,and proliferation of the protoscoleces.The results showed that the appropriate concentration of SB202190 for Em inhibition was 20 μmol/L.SB202190 increased Em Eosin staining rate and caspase-3 level,decreased EDU positive level and ROS level,OPN decreased Em Eosin staining rate and caspase-3 level,increased EDU positive rate and ROS level,and OPN decreased Em Eosin staining rate and caspase-3 level.Moreover,SB202190 can reverse the effect of OPN on Em.These results indicate that SB202190 can inhibit the activity and proliferation of Em,promote apoptosis,and be positively correlated with concentration,while OPN can promote the activity and proliferation of Em,inhibit apopto-sis,and reverse the effect of OPN on Em.These results provide evidence that OPN and p38MAPK signaling pathways may be new molecular targets for the treatment of alveolar echinococcosis.
万方数据
维普
