Isolation,Identification,Drug Resistance Analysis and Inhibition Effect of Bacillus subtilis of Clostridium perfringens from Chickens
In order to investigate the prevalence and drug resistance of Clostridium perfringens in the im-portant breeding area for yellow-feathered broilers,nine small intestinal samples with necrosis were ob-tained from diseased chickens in six different large-scale broiler farms in southern China(Guangdong,Guangxi province)and eastern China(Anhui,Fujian and Jiangsu province).All samples were collected for isolation,identification,toxin typing detection,drug resistance analysis,and agar diffusion assay for detec-ting the inhibitory effect of the field-isolated Bacillus subtilis BSGZ7 on C.perfringens.The results showed that seven strains of C.perfringens type A and 2 strains of type G from intestinal samples were i-solated,that all nine isolates and one standard strain were highly susceptible to amoxicillin,penicillin,cefo-taxime,ampicillin,cefalexin,flufenicol,and ceftriaxone.However,those were generally resistant to antimi-crobials such as gentamicin,kanamycin,tetracycline,doxycycline,streptomycin,sulfamisoxazole,lincomy-cin,erythromycin,and polymyxin B.All of the fermentation broths of B.subtilis BSGZ7 had an inhibitory effect on the ten strains of C.perfringens.In conclution,B.subtilis BSGZ7 exerts a certain antibacterial effect on C.perfringens,potentially serving as a novel,environmentally friendly,and safe antibacterial preparation for the prevention and control of necrotic enteritis in chickens.
Clostridium perfringensIsolation and identificationDrug susceptibility testBacillus subti-lisAntibacterial activity
万方数据
维普
