摘要
为探究产气荚膜梭菌在黄羽肉鸡重要养殖区域的流行及耐药情况,采集广东、广西等华南地区,安徽、江苏、福建等华东地区6个规模化肉鸡养殖场9份肠道病变样品进行分离鉴定和毒素分型检测、耐药性分析,琼脂扩散试验检测野外分离枯草芽孢杆菌BSGZ7对产气荚膜梭菌的抑菌效果.结果显示,从肠道样品中分离到7株A型和2株G型产气荚膜梭菌,9株分离菌和1株标准菌均对阿莫西林、青霉素、头孢噻肟、氨苄西林、头孢氨苄、氟苯尼考、头孢曲松具有较高的敏感性,对庆大霉素、卡那霉素、四环素、多西环素、链霉素、磺胺异恶唑、林可霉素、红霉素、多黏菌素B等抗菌药物普遍耐药,枯草芽孢杆菌BSGZ7发酵液均对10株菌有抑菌效果.结果表明枯草芽孢杆菌对产气荚膜梭菌具有一定抑菌作用,有望成为防控鸡坏死性肠炎的新型绿色安全的抗菌制剂,同时也为临床上鸡坏死性肠炎的防控和治疗提供了参考依据.
Abstract
In order to investigate the prevalence and drug resistance of Clostridium perfringens in the im-portant breeding area for yellow-feathered broilers,nine small intestinal samples with necrosis were ob-tained from diseased chickens in six different large-scale broiler farms in southern China(Guangdong,Guangxi province)and eastern China(Anhui,Fujian and Jiangsu province).All samples were collected for isolation,identification,toxin typing detection,drug resistance analysis,and agar diffusion assay for detec-ting the inhibitory effect of the field-isolated Bacillus subtilis BSGZ7 on C.perfringens.The results showed that seven strains of C.perfringens type A and 2 strains of type G from intestinal samples were i-solated,that all nine isolates and one standard strain were highly susceptible to amoxicillin,penicillin,cefo-taxime,ampicillin,cefalexin,flufenicol,and ceftriaxone.However,those were generally resistant to antimi-crobials such as gentamicin,kanamycin,tetracycline,doxycycline,streptomycin,sulfamisoxazole,lincomy-cin,erythromycin,and polymyxin B.All of the fermentation broths of B.subtilis BSGZ7 had an inhibitory effect on the ten strains of C.perfringens.In conclution,B.subtilis BSGZ7 exerts a certain antibacterial effect on C.perfringens,potentially serving as a novel,environmentally friendly,and safe antibacterial preparation for the prevention and control of necrotic enteritis in chickens.
维普
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