Drug Sensitivity Analysis of Clostridium perfringens from Different Host Sources before and after Biofilm Formation
To detect the biofilm formation ability and antimicrobial sensitivity of five strains of Clostridium perfringens from different hosts.Drug resistance of the five strains to seven antimicrobial was determined by the disk diffusion method.The minimum inhibitory concentration(MIC)of each strain was determined after screening the highly sensitive drugs.Crystal violet staining was used to detect the biofilm formation a-bility of C.perfringens from different sources and the biofilm inhibition and clearance ability of high-sensi-tivity drugs.Results showed that five strains of C.perfringens from different hosts were highly sensitive to ampicillin,and had varying degrees of resistance to the other six antimicrobial.The MIC values of ampicillin against different strains ranged from 0.031 µg/mL to 2.000 µg/mL.All strains could form biofilms,but the ability of biofilm formation was different.The biofilm formation of different strains was inhibited to differ-ent degrees at 4 MIC,2 MIC,and 1 MIC,respectively.While ampicillin at sub-inhibitory concentration could stimulate biofilm formation,ampicillin could not completely remove the biofilm that had already formed.The results provide some references for the selection of clinical drugs and the dosages of drugs.
万方数据
维普
