Application and Establishment of a Real-time PCR Detection Method for Pigeon Circovirus
To develop a rapid diagnostic method for pigeon circovirus(PiCV),a real-time PCR assay method for PiCV was established,using the primers and probe designed on the conserved sequence of Rep gene.The standard curve of the real-time PCR assay had good linear relationships in the range of 1.44X 101-1.44×107 copies/μL of template with the linear correlation coefficients to 0.986 6.The assay had a sensi-tivity of 1.44×10 copies/μL which showed that it had a good sensitivity.There was no cross-reaction with pigeon Newcastle disease virus,pigeon herpesvirus,pigeon adenovirus,pigeon salmonellosis and pigeon tis-sue DNA.This method has good repeatability,with coefficients of valuation ranging from 0.454％to 0.473％for intrabatch repeated experiments and 0.367％to 0.869％for interbatch repeated experiments.Compared with traditional PCR assay,the real-time PCR assay established in this study had better sensitiv-ity and slightly higher detection rate in clinical samples,with a detection coincidence rate 93.75％.In con-clusion,the real-time PCR assay performed as a diagnosis tool for PiCV detection,which provides a techni-cal support for the epidemiological investigation of PiCV.
万方数据
维普
