Effects of Swainsonine on Spleen Immune and Metabolism in Rabbits
This study was conducted to investigate the impact of natural swainsonine(SW)digested and absorbed through the digestive system on immunity and metabolism in rabbits.24 rabbits(1.9 kg±0.12 kg)were randomly assigned into an experimental group and a control group,with an equal distribution of 6 females and 6 males in each group.The experimental group was fed a homemade pellet feed containing a SW dosage of 2 mg SW/kg weight per day,while the control group was fed an equivalent weight of feed without locoweed.The animals were housed indi-vidually in cages and provided with ad libitum access to drinking water.Daily health inspections and recording were performed throughout the study period.On the 60th day,spleens were obtained from the rabbits for quantitative pro-teomics analysis using iTRAQ and bioinformatics.The results showed toxicity in all rabbits of the experimental group,with the identification of 315 differential proteins(＞1.5 or＜1/1.5)compared to the control.These proteins were primarily associated with metabolism(81％)and immunity(15％).Further analysis through Pathway Acti-vation Strength(PAS),Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)indicated that SW inhibited spleen immunity and activated spleen catabolic processes in rabbits exposed to SW toxicity,al-though metabolism was suppressed.This study provided evidence that long-term consumption of SW is detrimental to the development of spleen and the maintenance of a normal immune system in rabbits.The findings of this study suggest that caution should be taken when consuming SW-containing plants,and more attention should be paid to potential health risks.
万方数据
维普
