In order to reveal the infection status and distribution characteristics of Angiostrongylus cantonensis in freshwater snails in Yunnan province,a total of 4502 samples of 5 species of snails were collected from field and farm-ers'markets.The larvae of Angiostrongylus cantonensis were examined by tissue compression slide method,and tissue DNA was extracted.PCR was used to amplify the ITS gene of Angiostrongylus cantonensis.Microscopic examination showed that stage Ⅲ larvae of Angiostrongylus cantonensis were found in Pomacea canaliculata.PCR amplification showed that 5 samples were positive for Angiostrongylus cantonensis,the total positive rate was 0.111％(5/4 502).A-mong different regions,the positive rate was highest in Kunming(0.231％,4/1 734),followed by Dehong(0.221％,1/453).Among the different freshwater snail species,the positive rate of Pomacea canaliculata was 0.192％(4/2 081),the positive rate of Angiostrongylus cantonensis in Bellamya was 0.160％(1/624).However,Angiostrongylus can-tonensis infection was not detected in the other 3 snail species.Under different sampling environments,the positive rate was 0.192％(4/2 083)in the farmer's market and 0.041％(1/2 419)in the field environment.The infection rates of dif-ferent snail species and sampling environment were statistically significant(P＜0.05).In this study,we revealed the in-fection of Angiostrongylus cantonensis in some freshwater snails in Yunnan province,which can provided important basic data for the prevention and control of Angiostrongylus cantonensis in Yunnan province.
维普
万方数据