Investigation and Analysis of Angiostrongylus cantonensis Infection in Freshwater Snails in Yunnan Province
In order to reveal the infection status and distribution characteristics of Angiostrongylus cantonensis in freshwater snails in Yunnan province,a total of 4502 samples of 5 species of snails were collected from field and farm-ers'markets.The larvae of Angiostrongylus cantonensis were examined by tissue compression slide method,and tissue DNA was extracted.PCR was used to amplify the ITS gene of Angiostrongylus cantonensis.Microscopic examination showed that stage Ⅲ larvae of Angiostrongylus cantonensis were found in Pomacea canaliculata.PCR amplification showed that 5 samples were positive for Angiostrongylus cantonensis,the total positive rate was 0.111％(5/4 502).A-mong different regions,the positive rate was highest in Kunming(0.231％,4/1 734),followed by Dehong(0.221％,1/453).Among the different freshwater snail species,the positive rate of Pomacea canaliculata was 0.192％(4/2 081),the positive rate of Angiostrongylus cantonensis in Bellamya was 0.160％(1/624).However,Angiostrongylus can-tonensis infection was not detected in the other 3 snail species.Under different sampling environments,the positive rate was 0.192％(4/2 083)in the farmer's market and 0.041％(1/2 419)in the field environment.The infection rates of dif-ferent snail species and sampling environment were statistically significant(P＜0.05).In this study,we revealed the in-fection of Angiostrongylus cantonensis in some freshwater snails in Yunnan province,which can provided important basic data for the prevention and control of Angiostrongylus cantonensis in Yunnan province.
万方数据
维普
