Intestinal Ciliate Infection and Molecular Identification of Dairy Cows in Some Areas of Henan Province
In order to determine the infection of intestinal ciliates and the risk of zoonosis in dairy cows,462 fresh fecal samples were collected from 8 intensive dairy farms in different areas of Henan province.The infection and molecular characteristics of ciliates in the samples were identified by direct fecal smear microscopic examination and based on 18S rDNA and ITS1-5.8S rDNA-ITS2 sequences.The results showed that there were Buxtonella sulcata and Balantidioes coli in cow feces,in which the positive rate of Bu.sulcata was 29.9％.The positive rate of adult cattle was significantly higher than that of other ages(P＜0.01);The positive rate of Ba.coli was 3.9％,and there was no significant difference among different ages(P＞0.05),Eight samples were mixed infection of Bu.sulcata and Ba.coli.The rumen ciliates were not detected in the feces of dairy cows,indicating that the symbiotic ciliates in the rumen of dairy cows were not excreted with the feces.The results provide important basic data for the preven-tion and control of ciliosis in intensive dairy farms.
万方数据
维普
