首页|Zinc lactate alleviates oxidative stress by modulating crosstalk between constitutive androstane receptor signaling pathway and gut microbiota profile in weaned piglets

Zinc lactate alleviates oxidative stress by modulating crosstalk between constitutive androstane receptor signaling pathway and gut microbiota profile in weaned piglets

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This study aimed to determine the regulatory mechanism of dietary zinc lactate(ZL)supplementation on intestinal oxidative stress damage in a paraquat(PQ)-induced piglet model.Twenty-eight piglets(mean body weight 9.51±0.23 kg)weaned at 28 d of age were randomly divided into control,ZL,PQ,and ZL+PQgroups(n=7 in each group).The ZL-supplemented diet had little effect on growth performance under normal physiological conditions.However,under PQ challenge,ZL supplementation significantly improved average daily gain(P＜0.05)and reduced the frequency of diarrhea.ZL improved intestinal morphology and ultra-structure by significantly increasing the expression level of the jejunal tight junction protein,zonula occludens-1(ZO-1)(P＜0.05),and intestinal zinc transport and absorption in PQ-induced piglets,which reduced intestinal permeability.ZL supplementation also enhanced the expression of antioxidant and anti-inflammatory factor-related genes and decreased inflammatory cytokine expression and secretion in PQ-induced piglets.Furthermore,ZL treatment significantly inhibited the activation of constitutive androstane receptor(CAR)signaling(P＜0.01)in PQ-induced piglets and altered the structure of the gut microbiota,especially by significantly increasing the abundance of beneficial gut microbes,including UCG_002,Rumi-nococcus,Rikenellaceae_RC9gut_group,Christensenellaceae_R_7_group,Treponema,unclassified_Christense-nellaceae,and unclassified_Erysipelotrichaceae(P＜0.05).These data reveal that pre-administration of ZL to piglets can suppress intestinal oxidative stress by improving antioxidant and anti-inflammatory capacity and regulating the crosstalk between CAR signaling and gut microbiota.

Zinc lactateIntestinal barrierOxidative stressInflammationConstitutive androstane receptorGut microbiota

Wenjie Tang、Xuan Xiang、Houfu Wang、Wentao Zhou、Liuqin He、Yulong Yin、Tiejun Li

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College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China

Sichuan Academy of Animal Sciences,Animal Breeding and Genetics Key Laboratory of Sichuan Province,Chengdu 610066,China

Livestock and Poultry Biological Products Key Laboratory of Sichuan Province,Sichuan Animtech Feed Co.,Ltd,Chengdu 610000,China

Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process,CAS Key Laboratory of Agro-Ecological Processes in Subtropical Region,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China

College of Advanced Agricultural Science,University of Chinese Academy of Sciences,Beijing 100049,China

Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation,Laboratory of Animal Nutrition and Human Health,College of Life Sciences,Hunan

College of Advanced Agricultural Science,University of Chinese

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National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaHunan Key Research and Development PlanShandong Province Taishan Industry Leading Talents Project Blue Talents ProjectPublic Service Technology Center,Institute of Subtropical Agriculture,Chinese Academy of Sciences

32172755321300992022NK2023

2024

10.1016/j.aninu.2023.10.001

动物营养(英文)

动物营养(英文)

CSTPCD
ISSN：
年,卷(期)：2024.16(1)
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