Flavonoids from mulberry leaves inhibit fat production and improve fatty acid distribution in adipose tissue in finishing pigs

Yingying Liu ¹Yinglin Peng ²Chen Chen ²Huibo Ren ²Ji Zhu ²Yuan Deng ²Qingming Cui ²Xionggui Hu ²Jianhua He ³Huali Li ²Xinghui Zhu ⁴Yulong Yin ⁵Jun He ³Yi Xiao⁴

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作者信息

1. College of Animal Science and Technology,Hunan Agricultural University,Changsha,410128,China;Hunan Institute of Animal and Veterinary Science,Changsha,410131,China;Key Laboratory of Conservation and Genetic Analysis of Local Pig Breed Germplasm Resources,Changsha,410131,China
2. Hunan Institute of Animal and Veterinary Science,Changsha,410131,China;Key Laboratory of Conservation and Genetic Analysis of Local Pig Breed Germplasm Resources,Changsha,410131,China
3. College of Animal Science and Technology,Hunan Agricultural University,Changsha,410128,China
4. College of Information and Intelligence,Hunan Agricultural University,Changsha,410128,China
5. College of Animal Science and Technology,Hunan Agricultural University,Changsha,410128,China;Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,410125,China
折叠

Abstract

This study evaluated the effects of flavonoids from mulberry leaves(FML)on plasma biochemical indices,serum activities of lipid metabolism-related enzymes,fat morphology,fatty acid composition,and lipid metabolism in different adipose tissues of finishing pigs.We used 120 Chinese hybrid barrows of Berkshire and Bama mini-pigs with an average initial body weight of 45.11±4.23 kg.The pigs were randomly assigned to five treatment groups and fed a control diet based on corn,soybean meal,and wheat bran or a control diet supplemented with 0.02％,0.04％,0.08％,or 0.16％FML.Each experimental group had six replicates(pens),with four pigs per pen.After a 7-d adaptation period,the feeding trial was conducted for 58 d.Blood and adipose tissue samples were collected from 30 pigs(one pig per pen)at the end of the test.The results showed that FML supplementation significantly decreased the feed intake to body gain ratio,the plasma concentrations of total cholesterol and free fatty acids,and the serum activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase(linear or quadratic effects,P＜0.05),and decreased the plasma triglyceride concentration(quadratic,P=0.07).Increasing FML supplementation increased the average daily gain and serum activities of lipoprotein lipase(linear and quadratic effects,P＜0.05)and adipose triglyceride lipase(linear,P＜0.05).Dietary FML supplementation decreased the adipocyte area in the dorsal subcutaneous adipose(DSA)tissue of finishing pigs(linear,P=0.05)and increased the adipocyte area in the visceral adipose tissue(quadratic,P＜0.01).Increasing FML supplementation decreased the C20:1 content in DSA,abdominal subcutaneous adipose,and visceral adipose tissues of finishing pigs(P＜0.05)and increased the C18:3n3 and n-3 PUFA contents(P＜0.05).The lipid metabolism genes were regulated by the PPARy-LXRa-ABCA1 signaling pathway,and their expressions differed in different adipose tissues.These findings suggest that FML improved growth performance,regulated lipid metabolism,inhibited fat production,and improved fatty acid distribution in the adipose tissue of finishing pigs,thereby improving pig fat's nutritional quality and health value.

Key words

Finishing pig/Mulberry leaf/Flavonoid/Lipid metabolism/Fatty acid/Lard

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基金项目

Hunan Province Key R&D Program Project(2020NK2033)

Natural Science Foundation of Hunan Province(2022JJ30317)

Innovation Platform and Talent Plan Program of Hunan Province(2022NK4145)

National Natural science Foundation of China(31972600)

出版年

2024

动物营养(英文)

CSTPCD

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