动物营养(英文)2024，Vol.16Issue(1) ：158-173.DOI:10.1016/j.aninu.2023.10.005

Leucine regulates lipid metabolism in adipose tissue through adipokine-mTOR-SIRT1 signaling pathway and bile acid-microbe axis in a finishing pig model

Yunju Yin Saiming Gong Mengmeng Han Jingzun Wang Hanjing Shi Xianji Jiang Liu Guo Yehui Duan Qiuping Guo Qinghua Chen Fengna Li
动物营养(英文)2024，Vol.16Issue(1) ：158-173.DOI:10.1016/j.aninu.2023.10.005
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Leucine regulates lipid metabolism in adipose tissue through adipokine-mTOR-SIRT1 signaling pathway and bile acid-microbe axis in a finishing pig model

Yunju Yin 1Saiming Gong 1Mengmeng Han 2Jingzun Wang 3Hanjing Shi 4Xianji Jiang 1Liu Guo 2Yehui Duan 5Qiuping Guo 5Qinghua Chen 6Fengna Li2
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作者信息

  • 1. Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process,Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production,Changsha 410125,China;College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China
  • 2. Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process,Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production,Changsha 410125,China;College of Modern Agricultural Sciences,University of Chinese Academy of Sciences,Beijing 100049,China
  • 3. College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730124,China
  • 4. Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process,Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production,Changsha 410125,China;College of Life Sciences,Hunan Normal University,Changsha 410128,China
  • 5. Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process,Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production,Changsha 410125,China
  • 6. College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China
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Abstract

This study was conducted to explore the regulatory mechanism of leucine(Leu)on lipid metabolism of finishing pigs.Twenty-four Duroc x Landrace x Large cross pigs with an average body weight of 68.33±0.97 kg were randomly allocated into 3 treatment groups with 8 replicates per group(1 pig per replicate).The dietary treatments were as follows:control group(CON),0.25％Leu group and 0.50％Leu group.The experimental period was 42 d.The results showed as follows.(1)Compared with the CON,0.25％and 0.50％Leu increased(P＜0.01)the average daily gain(ADG),while the average backfat thickness(ABT)and the ratio of feed intake to body weight gain(F:G ratio)were decreased(P＜0.05).(2)In the 0.25％Leu group,the relative mRNA expression levels of sterol regulatory element binding protein-ic(SREBP1c),recombinant fatty acid transport protein 1(FATP1),chemerin and peroxisome proliferator-activated receptor γ(PPARγ)were decreased but the level of fatty acid binding protein 4(FABP4)and fatty acid translocase(FAT/CD36)were increased in backfat tissue.In the 0.25％Leu group,the protein levels of p-Rictor,p-Raptor,p-eIF4E-binding protein 1(p-4EBP1),p-silent mating type information regulator 2 homolog 1(p-SIRT1)and acetylation ribosome s6 protein kinase 1(Ac-S6K1)were increased(P＜0.05).(3)Compared to the CON,the diversity of gut microbiota in the 0.25％Leu group was increased.Principal component analysis showed that the relative abundance of Bacteroidetes,Lactobacillus and Desulfovibrio was higher in the 0.25％Leu group than the CON,but the relative abundance of Firmicutes,Treponema and Shigella was lower than in the CON(P＜0.05).(4)Four different metabolites were screened out from the serum of finishing pigs including allolithocholic acid(alloLCA),isolithocholic acid(isoLCA),urso-deoxycholic acid(UDCA)and hyodeoxycholic acid(HDCA),which correlate to various degrees with the above microorganisms.In conclusion,Leu could promote adipose tissue lipolysis of finishing pigs through the mTOR-SIRT1 signaling pathway,and S6K1 is acetylated at the same time,and the interaction between gut microbiota and bile acid metabolism is also involved.

Key words

Leu/Lipid metabolism/Gut microbiota/Bile acid

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基金项目

National Natural Science Foundation of China(31972582)

National Natural Science Foundation of China(U22A20516)

Science and Technology Innovation Program of Hunan Province(2021RC4039)

Key R & D Program of Hunan Province(2022NK2026)

Youth Innovation Promotion Association CAS(Y202079)

出版年

2024
动物营养(英文)

动物营养(英文)

CSTPCD
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