首页|Selenomethionine alleviates chronic heat stress-induced breast muscle injury and poor meat quality in broilers via relieving mitochondrial dysfunction and endoplasmic reticulum stress
Selenomethionine alleviates chronic heat stress-induced breast muscle injury and poor meat quality in broilers via relieving mitochondrial dysfunction and endoplasmic reticulum stress
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In the present study,the chronic heat stress(CHS)broiler model was developed to investigate the po-tential protection mechanism of organic selenium(selenomethionine,SeMet)on CHS-induced skeletal muscle growth retardation and poor meat quality.Four hundred Arbor Acres male broilers(680±70 g,21 d old)were grouped into 5 treatments with 8 replicates of 10 broilers per replicate.Broilers in the control group were raised in a thermoneutral environment(22±2 ℃)and fed with a basal diet.The other four treatments were exposed to hyperthermic conditions(33±2 ℃,24 h in each day)and fed on the basal diet supplied with SeMet at 0.0,0.2,0.4,and 0.6 mg Se/kg,respectively,for 21 d.Results showed that CHS reduced(P＜0.05)the growth performance,decreased(P＜0.05)the breast muscle weight and impaired the meat quality of breast muscle in broilers.CHS induced protein metabolic disorder in breast muscle,which increased(P＜0.05)the expression of caspase 3,caspase 8,caspase 9 and ubiquitin proteasome system related genes,while decreased the protein expression of P-4EBP1.CHS also decreased the antioxidant capacity and induced mitochondrial stress and endoplasmic reticulum(ER)stress in breast muscle,which increased(P＜0.05)the ROS levels,decreased the concentration of ATP,increased the protein expression of HSP60 and CLPX,and increased(P＜0.05)the expression of ER stress bio-markers.Dietary SeMet supplementation linearly increased(P＜0.05)breast muscle Se concentration and exhibited protective effects via up-regulating the expression of the selenotranscriptome and several key selenoproteins,which increased(P＜0.05)body weight,improved meat quality,enhanced antiox-idant capacity and mitigated mitochondrial stress and ER stress.What's more,SeMet suppressed protein degradation and improved protein biosynthesis though inhibiting the caspase and ubiquitin proteasome system and promoting the mTOR-4EBP1 pathway.In conclusion,dietary SeMet supplementation in-creases the expression of several key selenoproteins,alleviates mitochondrial dysfunction and ER stress,improves protein biosynthesis,suppresses protein degradation,thus increases the body weight and improves meat quality of broilers exposed to CHS.
Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education,of China Ministry of Agriculture and Rural Affairs,of Sichuan Province,Animal Nutrition Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China
College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130,Sichuan,China
National Natural Science Foundation of ChinaSpecial Research Funding for Discipline Construction in Sichuan Agricultural University
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