首页|The AMPK and AKT/GSK3β pathways are involved in recombinant proteins fibroblast growth factor 1(rFGF1 and rFGF1a)improving glycolipid metabolism in rainbow trout(Oncorhynchus mykiss)fed a high carbohydrate diet
The AMPK and AKT/GSK3β pathways are involved in recombinant proteins fibroblast growth factor 1(rFGF1 and rFGF1a)improving glycolipid metabolism in rainbow trout(Oncorhynchus mykiss)fed a high carbohydrate diet
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
Fibroblast growth factor 1(FGF1)regulates vertebrate cell growth,proliferation and differentiation,and energy metabolism.In this study,we cloned rainbow trout(Oncorhynchus my kiss)fgf1 and fgf1a,pre-pared their recombinant proteins(rFGF1 and rFGF1a),and described the molecular mechanisms by which they improve glycolipid metabolism in carnivorous fish.A 31-d feeding trial was conducted to investigate whether they could enhance glycolipid metabolism in rainbow trout on high-carbohydrate diets(HCD).A total of 720 rainbow trout(8.9±0.5 g)were equally divided into 4 groups:the chow diet(CD)group injected with PBS,the HCD group injected with PBS,the HCD group injected with rFGF1(400 ng/g body weight),and the HCD group injected with rFGF1a(400 ng/g body weight).The results showed that short-term HCD had a significant positive effect on the specific growth rate(SGR)of rainbow trout(P＜0.05).However,it led to an increase in crude fat,serum triglyceride(TG)and glucose content,as well as serum glutamic pyruvic transaminase(GPT)and glutamic oxalacetic transaminase(GOT)contents(P＜0.05),suggesting a negative health effect of HCD.Nevertheless,rFGF1 and rFGF1a showed beneficial therapeutic effects.They significantly reduced the crude fat content of the liver,serum TG,GOT,and GPT contents caused by HCD(P＜0.05).The upregulation in atgl,hsl,and acc2 mRNAs implied the promotion of TG catabolism.Moreover,rFGF1 and rFGF1a contributed to promoting lipolysis by activating the AMPK pathway and reducing lipid accumulation in the liver caused by HCD.In addition,the rFGF1 and rFGF1 a-treated groups significantly reduced serum glucose levels and elevated hepatic glycogen content under HCD,and increased glucose uptake by hepatocytes.We observed a decrease in mRNA levels for pepck,g6pase,and pygl,along with an increase in mRNA levels for gys,glut2,and gk in the liver.Furthermore,these proteins regulated hepatic gluconeogenesis and glycogen synthesis by increasing the phosphorylation level of AKT,ultimately leading to an increase in GSK3β phosphorylation.In conclusion,this study demonstrates that rFGF1 and rFGF1a can enhance lipolysis and glucose utili-zation in rainbow trout by activating the AMPK pathway and AKT/GSK3β axis.
NETL
NSTL
万方数据
