Trimethylamine oxide supplementation differentially regulates fat deposition in liver,longissimus dorsi muscle and adipose tissue of growing-finishing pigs

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外文摘要：Trimethylamine oxide(TMAO)is a microbiota-derived metabolite,and numerous studies have shown that it could regulate fat metabolism in humans and mice.However,few studies have focused on the effects of TMAO on fat deposition in growing-finishing pigs.This study aimed to investigate the effect of TMAO on fat deposition and intestinal microbiota in growing-finishing pigs.Sixteen growing pigs were randomly divided into 2 groups and fed with a basal diet with 0 or 1 g/kg TMAO for 149 d.The intestinal microbial profiles,fat deposition indexes,and fatty acid profiles were measured.These results showed that TMAO supplementation had a tendency to decrease lean body mass(P＜0.10)and significantly increased backfat thickness(P＜0.05),but it did not affect growth performance.TMAO significantly increased total protein(TP)concentration,and reduced alkaline phosphatase(ALP)concentration in serum(P＜0.05).TMAO increased the α diversity of the ileal microbiota community(P＜0.05),and it did not affect the colonic microbial community.TMAO supplementation significantly increased acetate content in the ileum,and Proteobacteria and Escherichia-Shigella were significantly enriched in the TMAO group(P＜0.05).In addition,TMAO decreased fat content,as well as the ratio of linoleic acid,n-6 polyunsaturated fatty acids(PUFA),and PUFA in the liver(P＜0.05).On the contrary,TMAO increased intramuscular fat content of the longissimus dorsi muscle,whereas the C18:2n6c ratio was increased,and the n-6 PUFA:PUFA ratio was decreased(P＜0.05).In vitro,1 mmol/LTMAO treatment significantly upregulated the expression of FASN and SREBP1 in C2C12 cells(P＜0.05).Nevertheless,TMAO also increased adipocyte area and decreased the CPT-1B expression in subcutaneous fat(P＜0.05).Taken together,TMAO supplementation regulated ileal microbial composition and acetate production,and regulated fat distribution and fatty acid composition in growing-finishing pigs.These results provide new insights for understanding the role of TMAO in humans and animals.

外文关键词：

Growing-finishing pigTrimethylamine oxideFat depositionFatty acid compositionIntestinal microbiota

作者：

Andong Zha、Wanquan Li、Jing Wang、Ping Bai、Ming Qi、Peng Liao、Bie Tan、Yulong Yin

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作者单位：

Laboratory of Animal Nutritional Physiology and Metabolic Process,Key Laboratory of Agro-ecological Processes in Subtropical Region,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China

University of Chinese Academy of Sciences,Beijing 100008,China

College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China

Yunnan Southwest Agriculture and Animal Husbandry Group Co.,Ltd.,Kunming 650224,China

Yunnan Southwest Agricult

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基金：

National Natural Science Foundation of Chinaearmarked fund for China Agriculture Research SystemScience and Technology Major Project of Yunnan ProvinceLargescale Healthy Breeding Technology Research and Industrialization Demonstration of pig

项目编号：

U20A2054CARS-35202202AE090032202102AE090046

出版年：

2024

DOI：

10.1016/j.aninu.2023.12.006

动物营养(英文)

CSTPCD

ISSN：

年,卷(期)：2024.17(2)