首页|Gut microbiota-bile acid crosstalk and metabolic fatty liver in spotted seabass(Lateolabrax maculatus):The role of a cholesterol,taurine and glycine supplement

Gut microbiota-bile acid crosstalk and metabolic fatty liver in spotted seabass(Lateolabrax maculatus):The role of a cholesterol,taurine and glycine supplement

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
The prevalent practice of substituting fishmeal with plant protein frequently leads to disturbances in bile acid metabolism,subsequently increasing the incidence of metabolic liver diseases.Bile acid nutrients such as cholesterol,taurine and glycine have been shown to enhance bile acid synthesis and confer beneficial effects on growth.Therefore,this study aimed to investigate the effects of cholesterol-taurine-glycine(Ch-Tau-Gly)supplement on bile acid metabolism and liver health in spotted seabass(Lateolabrax maculatus)fed a plant-based diet.Two isonitrogenous and isolipidic diets were formulated:(1)plant protein-based diet(PP);(2)PP supplemented 0.5％cholesterol,0.5％taurine and 1.3％glycine(CTG).Each experimental diet was randomly fed to quadruplicate groups of 30 feed-trained spotted seabass in each tank.The results revealed that supplementing plant-based diet with Ch-Tau-Gly supplement led to an increase in carcass ratio(meat yield)in spotted seabass(P＜0.05),indirectly contributing positively to their growth.The dietary supplement effectively suppressed endogenous cholesterol synthesis in the liver,promoted the expression of bile acid synthesis enzyme synthesis,and simultaneously the expression of intestinal fxr and its downstream genes,including hnf4α and shp(P＜0.05).The reduction in Lactobacillus_salivarius and bile salt hydrolase(BSH)were observed in CTG group with concurrently increased conjugated chenodeoxycholic acid(CDCA)bile acids(P＜0.05),suggesting the enhancement of the hydrophilicity of the bile acid pool.In CTG group,fatty liver was alleviated with a corresponding increase in lipid metabolism,characterized by a downregulation of genes associated with lipogenesis and lipid droplet deposition,along with an upregulation of genes related to lipolysis.Our study underscored the ability of Ch-Tau-Gly supplement to influence the gut microbiota,leading to an increase in the levels of conjugated CDCA(P＜0.05)in the bile acid pool of spotted seabass.The interplay between the gut microbiota and bile acids might constitute a crucial pathway in the promotion of liver health.These findings offer a promising solution,suggesting that Ch-Tau-Gly supplement have the potential to pro-mote the growth of aquatic species and livestock fed on plant-based diets while addressing issues related to metabolic fatty liver.

Cholesterol-taurine-glycine supplementPlant-based dietsFatty liverGut microbiota-bile acid crosstalkLipid metabolism

Tingting Song、Xiaofang Liang、Hao Wang、Min Xue、Jie Wang

展开 >

National Aquafeed Safety Assessment Center,Institute of Feed Research,Chinese Academy of Agricultural Sciences,Beijing 100081,China

National Key Research and Development Program of ChinaXinjiang Key Research and Development ProgramChina Postdoctoral Science Foundation

2022YFD13006002022B02039-32021M703544

2024

10.1016/j.aninu.2024.03.008

动物营养(英文)

动物营养(英文)

CSTPCD
ISSN：
年,卷(期)：2024.17(2)