首页|Next-generation probiotic candidates targeting intestinal health in weaned piglets:Both live and heat-killed Akkermansia muciniphila prevent pathological changes induced by enterotoxigenic Escherichia coli in the gut
Next-generation probiotic candidates targeting intestinal health in weaned piglets:Both live and heat-killed Akkermansia muciniphila prevent pathological changes induced by enterotoxigenic Escherichia coli in the gut
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The use of next-generation probiotics(NGP)in pigs for combating diseases has been subject to limited research.Here we explored the potential of a well-known NGP candidate Akkermansia muciniphila tar-geting pig gut health.In the first screening experiment,we found that the abundance of A.muciniphila peaked at 14 d old but decreased at weaning(21 d old;P＜0.05),suggesting the weaning period may be an effective window for A.muciniphila intervention.Following that,48 crossbred weaned pigs at 28 d old were randomly assigned to five groups:control(CON),high/low live A.muciniphila(HA/LA),and high/low heat-killed A.muciniphila(HIA/LIA).From 1 to 28 d old,the CON group received gastric infusion of anaerobic sterile saline every other day;the HA and LA groups were gavaged every other day with 1 × 1010 CFU/5 mL and 5 × 108 CFU/5 mL live A.muciniphila,respectively;and the HIA and LIA groups were gavaged every other day with 1 × 1010 CFU/5 mL and 5 × 108 CFU/5 mL heat-killed A.muciniphila,respectively.At d 29,pigs in the CON group were randomly and equally divided into two groups,one of which was named the enterotoxigenic Escherichia coli(ETEC)group,and all groups except CON received a 5-d ETEC challenge.The supplementation of A.muciniphila numerically reduced the diarrhea rate of weaned pigs compared to the pigs that only received the ETEC challenge(P=0.57),but the LIA group had a higher diarrhea rate than the CON group(P＜0.05).Consistent with this,the supplementation of A.muciniphila improved the small intestinal morphology and structure,proportion of CD4+T lymphocytes in the blood,as well as the expression of genes related to intestinal barrier and antioxidant indices of pigs with ETEC challenge,especially for the LA group(P＜0.05).Meanwhile,A.muciniphila supplementation reduced the expression of ETEC virulence factor genes in the ileum and colon of pigs challenged by ETEC(P＜0.05).Therefore,A.muciniphila may protect the intestinal health of weaned piglets from damage caused by ETEC infection,but the effect may vary depending on the concentration and activity of A.muciniphila.
Akkermansia muciniphilaWeaned pigletEnterotoxigenic Escherichia coli challengeIntestinal health
Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education of China,Key Laboratory for Animal Disease-Resistance Nutrition and Feed of Ministry of Agriculture of China,Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province,Engineering Research Center of Animal Disease-Resistance Nutrition Biotechnology of Ministry of Education of China,Animal Nutrition Institute,Sichuan Agricultural University,Chengdu 611100,China
Sichuan Provincial Natural Science FoundationNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNanjing Agricultural University for donating A.muciniphila
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