Effects of hydroxyproline supplementation in low fish meal diets on collagen synthesis,myofiber development and muscular texture of juvenile Pacific white shrimp(Litopenaeus vannamei)

Menglin Shi ¹Haoming Li ¹Tianyu Chen ¹Bocheng Huang ¹Xiaoyue Li ¹Xiaohui Dong ²Shuyan Chi ²Qihui Yang ²Hongyu Liu ²Junming Deng ²Beiping Tan ²Shuang Zhang ²Shiwei Xie³

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作者信息

1. Laboratory of Aquatic Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China
2. Laboratory of Aquatic Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province,Zhanjiang 524088,China;Key Laboratory of Aquatic,Livestock and Poultry Feed Science and Technology in South China,Ministry of Agriculture,Zhanjiang 524088,China
3. Laboratory of Aquatic Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province,Zhanjiang 524088,China;Key Laboratory of Aquatic,Livestock and Poultry Feed Science and Technology in South China,Ministry of Agriculture,Zhanjiang 524088,China;Guangdong Provincial Key Lab of Aquatic Animals Disease Control and Healthy Culture,Zhanjiang 524088,China
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Abstract

This experiment aimed to evaluate the impact of dietary hydroxyproline(Hyp)supplementation on the muscle quality of juvenile Pacific white shrimp(Litopenaeus vannamei)fed a low fishmeal diet.Six formulated diets included one high fishmeal(HF;25％fishmeal content)and five low fishmeal diets(10％fishmeal content)with 0％,0.2％,0.4％,0.6％and 0.8％Hyp(LFO,LF2,LF4,LF6 and LF8,respectively).Each diet was assigned to four replicates,and 40 shrimp(0.32±0.00 g)per replicate were fed four times a day for 8 weeks.Dietary Hyp supplementation had little effects on growth performance,but increased the contents of Hyp,prolyl 4-hydroxylases(P4Hs),and collagen.The meat yield,springiness,hardness,chewiness,and cohesiveness of muscle were the highest in the LF4 group among the low fishmeal groups(P＜0.05).Cooking loss and freezing loss of muscle were the lowest in the LF4 group(P＜0.05).Dietary supplementation with 0.4％Hyp increased the myofiber density and decreased the myofiber diameter of muscle(P＜0.05).Supplementation of Hyp in the diet up-regulated the mRNA expression of smyhc5,smyhc15,col1a1,col1a2,igf-1f,tgf-β and tor and down-regulated the mRNA expression of smy hc1,smyhc2,smyhc6a(P＜0.05).Supplementation of Hyp in the diet up-regulated the protein expression of P-4E-BP1,P-AKT,AKT and P-AKT/AKT(P＜0.05).These results suggested that the addition of 0.4％Hyp to low fishmeal diets improved the muscle quality of L.vannamei.

Key words

Hydroxyproline/Collagen/Muscle quality/Litopenaeus vannamei

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基金项目

National Natural Science Foundation of China(32373141)

Guangdong Basic and Applied Basic Research Foundation(2023A1515030007)

ModernSeed Industry Park for Whiteleg Shrimp of Guangdong Province()

program for scientific research startup funds of Guangdong Ocean University,Science and Technology Bureau of Zhanjiang(2020A05003)

program for scientific research startup funds of Guangdong Ocean University,Science and Technology Bureau of Zhanjiang(2022A 01002)

出版年

2024

动物营养(英文)

CSTPCD

ISSN：

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