首页|Effects of hydroxyproline supplementation in low fish meal diets on collagen synthesis,myofiber development and muscular texture of juvenile Pacific white shrimp(Litopenaeus vannamei)
Effects of hydroxyproline supplementation in low fish meal diets on collagen synthesis,myofiber development and muscular texture of juvenile Pacific white shrimp(Litopenaeus vannamei)
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This experiment aimed to evaluate the impact of dietary hydroxyproline(Hyp)supplementation on the muscle quality of juvenile Pacific white shrimp(Litopenaeus vannamei)fed a low fishmeal diet.Six formulated diets included one high fishmeal(HF;25％fishmeal content)and five low fishmeal diets(10％fishmeal content)with 0％,0.2％,0.4％,0.6％and 0.8％Hyp(LFO,LF2,LF4,LF6 and LF8,respectively).Each diet was assigned to four replicates,and 40 shrimp(0.32±0.00 g)per replicate were fed four times a day for 8 weeks.Dietary Hyp supplementation had little effects on growth performance,but increased the contents of Hyp,prolyl 4-hydroxylases(P4Hs),and collagen.The meat yield,springiness,hardness,chewiness,and cohesiveness of muscle were the highest in the LF4 group among the low fishmeal groups(P＜0.05).Cooking loss and freezing loss of muscle were the lowest in the LF4 group(P＜0.05).Dietary supplementation with 0.4％Hyp increased the myofiber density and decreased the myofiber diameter of muscle(P＜0.05).Supplementation of Hyp in the diet up-regulated the mRNA expression of smyhc5,smyhc15,col1a1,col1a2,igf-1f,tgf-β and tor and down-regulated the mRNA expression of smy hc1,smyhc2,smyhc6a(P＜0.05).Supplementation of Hyp in the diet up-regulated the protein expression of P-4E-BP1,P-AKT,AKT and P-AKT/AKT(P＜0.05).These results suggested that the addition of 0.4％Hyp to low fishmeal diets improved the muscle quality of L.vannamei.
Laboratory of Aquatic Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China
Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province,Zhanjiang 524088,China
Key Laboratory of Aquatic,Livestock and Poultry Feed Science and Technology in South China,Ministry of Agriculture,Zhanjiang 524088,China
Guangdong Provincial Key Lab of Aquatic Animals Disease Control and Healthy Culture,Zhanjiang 524088,China
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National Natural Science Foundation of ChinaGuangdong Basic and Applied Basic Research FoundationModernSeed Industry Park for Whiteleg Shrimp of Guangdong Provinceprogram for scientific research startup funds of Guangdong Ocean University,Science and Technology Bureau of Zhanjiangprogram for scientific research startup funds of Guangdong Ocean University,Science and Technology Bureau of Zhanjiang
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