Dietary copper improves intestinal structural integrity in juvenile grass carp(Ctenopharyngodon idella)probably related to its increased intestinal antioxidant capacity and apical junction complex

Rui Ma ¹Lin Feng ²Pei Wu ²Yang Liu ²Hong-Mei Ren ¹Xiao-Wan Jin ¹Shu-Wei Li ³Ling Tang ³Xiao-Qiu Zhou ²Wei-Dan Jiang²

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作者信息

1. Animal Nutrition Institute,Sichuan Agricultural University,Chengdu 611130,China
2. Animal Nutrition Institute,Sichuan Agricultural University,Chengdu 611130,China;Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province,Sichuan Agricultural University,Chengdu 611130,China;Key Laboratory of Animal Disease-Resistance Nutrition,Ministry of Education,Ministry of Agriculture and Rural Affairs,Key Laboratory of Sichuan Province,Chengdu 611130,China
3. Animal Nutrition Institute,Sichuan Academy of Animal Science,Sichuan Animtech Feed Co.Ltd.,Chengdu 610066,China
折叠

Abstract

This research evaluated the effects of copper(Cu)on intestinal antioxidant capacity and apical junctional complex(AJC)in juvenile grass carp.A total of 1080 healthy juvenile grass carp(11.16±0.01 g)were fed six diets including different dosages of Cu,namely 0,2,4,6,8 mg/kg(Cu citrate[CuCit]as Cu source)and 3 mg/kg(CuSO4-5H2O as Cu source).The trial lasted for 9 weeks.The findings revealed that dietary optimal Cu supplementation(2.2 to 4.1 mg/kg)promoted intestinal growth,including intestinal length,intestinal length index,intestinal weight,and intestinal somatic index(P＜0.05).Furthermore,optimal Cu boosted the intestinal mucosal barrier in juvenile grass carp.On the one hand,optimal Cu reduced diamine oxidase and D-lactate levels in serum(P＜0.05),reduced levels of the oxidative damage in-dicators malondialdehyde,reactive oxygen species(ROS),protein carbonyl,superoxide dismutase(P＜0.05),and catalase mRNA levels were elevated(P＜0.05),thus boosting intestinal antioxidant ca-pacity,the binding protein Keap1a/1b/Nrf2 signaling pathway might be involved.Optimal Cu had no impact on glutathione peroxidase 1b(GPx1b)gene expression(P＞0.05).On the other hand,optimal Cu increased intestinal tight junction(TJ)proteins(except for claudin 15b)and adherens junction(AJ)proteins(E-cadherin,α-catenin,β-catenin,nectin and afadin)mRNA levels(P＜0.05),which could be connected to the signaling pathway formed by the Ras homolog gene family,member A(RhoA),Rho-associated kinase(ROCK),and myosin light chain kinase(MLCK).Finally,based on serum indicator D-lactate and intestinal oxidative damage index(ROS),Cu requirement(CuCit as Cu source)for juvenile grass carp from initial weight to final weight(from 11 to 173 g)was determined to be 4.14 and 4.12 mg/kg diet,respectively.This work may provide a theoretical foundation for identifying putative Cu regulation pathways on fish intestinal health.

Key words

Copper/Ctenopharyngodon idella/Structural integrity/Intestine

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基金项目

earmarked fund for CARS(CARS-45)

National Guiding Local Science and Technology Development Fund Projects(23ZYZYTS0513)

National Key R&D Program of China(2019YFD0900200)

National Natural Science Foundation of China for Outstanding Youth Science Foundation(31922086)

出版年

2024

动物营养(英文)

CSTPCD

ISSN：

段落导航