Activation of skeletal carbohydrate-response element binding protein(ChREBP)-mediated de novo lipogenesis increases intramuscular fat content in chickens

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外文摘要：The intracellular lipids in muscle cells of farm animals play a crucial role in determining the overall intramuscular fat(IMF)content,which has a positive impact on meat quality.However,the mechanisms underlying the deposition of lipids in muscle cells of farm animals are not yet fully understood.The purpose of this study was to determine the roles of carbohydrate-response element binding protein(ChREBP)and fructose in IMF deposition of chickens.For virus-mediated ChREBP overexpression in tibialis anterior(TA)muscle of chickens,seven 5-d-old male yellow-feather chickens were used.At 10 d after virus injection,the chickens were slaughtered to obtain TA muscles for analysis.For fructose administration trial,sixty 9-wk-old male yellow-feather chickens were randomly divided into 2 groups,with 6 replicates per group and 5 chickens per replicate.The chickens were fed either a basal diet or a basal diet supplemented with 10％fructose(purity ≥ 99％).At 4 wk later,the chickens were slaughtered,and breast and thigh muscles were collected for analysis.The results showed that the skeletal ChREBP mRNA levels were positively associated with IMF content in multiple species,including the chickens,pigs,and mice(P＜0.05).ChREBP overexpression increased lipid accumulation in both muscle cells in vitro and the TA muscles of mice and chickens in vivo(P＜0.05),by activation of the de novo lipo-genesis(DNL)pathway.Moreover,activation of ChREBP by dietary fructose administration also resulted in increased IMF content in mice and notably chickens(P＜0.05).Furthermore,the lipidomics analysis revealed that ChREBP activation altered the lipid composition of chicken IMF and tented to improve the flavor profile of the meat.In conclusion,this study found that ChREBP plays a pivotal role in mediating the deposition of fat in chicken muscles in response to fructose-rich diets,which provides a novel strategy for improving meat quality in the livestock industry.

外文关键词：

ChickenMeat qualityIntramuscular fatCarbohydrate-response element binding proteinFructose

作者：

Peng Wang、Haihan Xiao、Tian Wu、Qinghua Fu、Xudong Song、Yameng Zhao、Yan Li、Jieping Huang、Ziyi Song

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作者单位：

Guangxi Key Laboratory of Animal Breeding,Disease Control and Prevention,College of Animal Science and Technology,Guangxi University,Nanning,Guangxi 530004,China

基金：

Specific Research Project of Guangxi for Research Bases and TalentsNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaProject of Bama County for Talents in Science and Technology

项目编号：

AD22035061821009138236016620220016

出版年：

2024

DOI：

10.1016/j.aninu.2024.04.006

动物营养(英文)

CSTPCD

ISSN：

年,卷(期)：2024.18(3)