Combined intestinal microbiota and transcriptomic analysis to investigate the effect of different stocking densities on the ability of Pacific white shrimp(Litopenaeus vannamei)to utilize Chlorella sorokiniana

扫码查看

原文链接

NETL
NSTL
万方数据

外文摘要：Aiming to investigate the impact of different stocking densities on the ability of Pacific white shrimp(Litopenaeus vannamei)to utilize Chlorella sorokiniana(CHL),a 3 × 2 factorial design stocking experiment was used in this study.Specifically,shrimp was fed with two dietary protein sources(fishmeal[FM]and CHL)at low(LSD;100 per m3),medium(MSD;200 per m3)and high(HSD;300 per m3)stocking densities for 8 weeks.The growth performance and resistance to Vibrio parahaemolyticus(1.0 × 107 CFU/mL)of shrimp decreased with the increase of stocking density,but dietary CHL improved this result.Differences between the CHL and FM groups for V.parahaemolyticus resistance were significant only under high-density conditions(P＜0.05).Significant interactions between stocking density and protein source were found on the activities of catalase(CAT),superoxide dismutase(SOD)and phenol oxidase(PO),and the contents of malondialdehyde(MDA)in the hepatopancreas and the activities of intestinal amylase,most of which were significantly different between CHL and FM groups only at high stocking density(P＜0.05).Analysis of 16S rDNA sequencing showed that dietary CHL increased the alpha di-versity of intestinal microbiota,inhibited the colonization of pathogenic bacteria and enhanced the abundance of beneficial bacteria.Transcriptomic results showed that at high stocking densities,differ-entially expressed genes(DEGs)in the FM vs CHL group were mostly upregulated and primarily enriched in immune and metabolic related pathways including Toll,immune deficiency(Imd)and glycolysis-gluconeogenesis pathways.Pearson correlation analysis revealed significant correlation between the top ten intestinal bacteria at the genus level and markedly enriched DEGs,also more were detected under high density situations.In conclusion,CHL has great potential as a novel protein source in the intensive farming of shrimp.

外文关键词：

Chlorella sorokinianaStocking densityMicrobiota structureTranscriptomic profileLitopenaeus vannamei

作者：

Hang Yuan、Minghua Xie、Jian Chen、Naijie Hu、Honming Wang、Beiping Tan、Lili Shi、Shuang Zhang

展开 >

作者单位：

College of Fisheries,Guangdong Ocean University,Zhanjiang,China

College of Fisheries and Life Science,Dalian Ocean University,Dalian,China

Zhanjiang Experimental Station,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang,China

基金：

Special Projects in Key Fields of Ordinary University in Guangdong ProvinceGeneral Program of Natural Science Foundation of Guangdong Province,ChinaPostgraduate Education Innovation Project of Guangdong Ocean University in 2023

项目编号：

2023ZDZX40102022A1515012325202336

出版年：

2024

DOI：

10.1016/j.aninu.2024.04.008

动物营养(英文)

CSTPCD

ISSN：

年,卷(期)：2024.18(3)