Abstract
This study investigated the impact of different ratios of soluble to insoluble dietary fiber(SDF:IDF)for-mulations by sugar beet pulp(SBP)supplementation on piglet growth performance,nutrient di-gestibility,immune function,intestinal morphology,intestinal microbiota and intestinal health.A total of 60 crossbred piglets(Duroc ×[Landrace × Yorkshire])at 40 d old with body weight of 10.0±0.3 kg were randomly assigned to 5 treatments with 6 replicates per treatment and 2 piglets per replicate in a 21-d trial.The dietary treatments included a corn-soybean meal diet(0％SBP supplementation;CON),and diets supplemented with 2％,4％,6％,and 8％SBP,representing different SDF:IDF ratios at 10.16％,13.53％,16.79％,19.86％,and 24.81％,respectively.The results indicated that the 8％SBP treatment had a negative effect on feed-to-gain ratio(linear,P=0.009)compared with the CON treatment(P=0.021).The apparent total tract digestibility(ATTD)of crude protein was lower in treatments supplemented with SBP(P=0.002)and showed a linear decrease(P=0.001),while the ATTD of IDF showed a linear increase(P=0.037)in four SBP treatments compared to the CON treatment.The 4％SBP treatment increased serum concentrations of triglyceride(quadratic,P=0.019)and K(linear,P=0.037),and decreased alanine transaminase concentration(quadratic,P=0.015)compared with the CON treatment.The concentrations of Cit,Cys,Ile,Leu,Orn,Arg,taurine,urea,1-methylhistidine,α-aminoadipic acid,α-aminobutyric acid and cystathionine in the 4％SBP treatment were highest among all treatments(P＜0.05).The serum concentrations of interleukin-6,interleukin-8,interleukin-10,transforming growth factor-β,and tumor necrosis factor-α in the 6％SBP treatment were higher than those in the CON treatment(P＜0.05),which also increased mucin-2 and G protein-coupled receptor 41 mRNA expression(P＜0.05)in colonic mucosa compared with the CON treatment and improved the intestinal barrier function.Diets containing more than 19.86％SDF:IDF could impair the intestinal health in piglets when SBP was used as the SDF source.Supplementing nursery piglet diets with 16.79％to 19.86％SDF:IDF is recommended for improving intestinal barrier function,increasing short-chain fatty acids concentra-tions,and improving intestinal microbiota composition.
基金项目
National Natural Science Foundation of China(U20A2054)
Natural Science Foundation of Hunan Province(2024JJ1004)
Science and Technology Major Project of Yunnan Province(202202AE090032)
NETL
NSTL
万方数据