Abstract
Dietary fat content can reduce the methane production of dairy cows;however,the relevance fatty acid(FA)composition has towards this inhibitory effect is debatable.Furthermore,in-depth studies eluci-dating the effects of unsaturated fatty acids(UFA)on rumen function and the mechanism of reducing methane(CH4)production are lacking.This study exposed 10 Holstein cows with the same parity,similar milk yield to two total mixed rations:low unsaturated FA(LUFA)and high unsaturated FA(HUFA)with similar fat content.The LUFA group mainly added fat powder(C16∶0＞90％),and the HUFA group mainly replaced fat powder with extruded flaxseed.The experiment lasted 26 d,the last 5 d of which,gas exchange in respiratory chambers was conducted to measure gas emissions.We found that an increase in the UFA in diet did not affect milk production(P＞0.05)and could align the profile of milk FAs more closely with modern human nutritional requirements.Furthermore,we found that increasing the UFA content in the diet lead to a decrease in the abundance of Methanobrevibacter in the rumen(|linear discriminant analysis[LDA]score|＞2 and P＜0.05),which resulted in a decrease in the relative abundance of multiple enzymes(EC:1.2.7.12,EC:2.3.1.101,EC:3.5.4.27,EC:1.5.98.1,EC:1.5.98.2,EC:6.2.1.1,EC:2.1.1.86 and EC:2.8.4.1)during methanogenesis(P＜0.05).Compared with the LUFA group,the pathway of CH4 metabolism was inhibited in the HUFA group(|LDA|＞2 and P＜0.05),which ultimately decreased CH4 production(P＜0.05).Our results illustrated the mechanism involving decreased CH4 production when fed a UFA diet in dairy cows.We believe that our study provides new evidence to explore CH4 emission reduction measures for dairy cows.
基金项目
National Key R&D Program of China(2022YFD1301001)
NETL
NSTL
万方数据