首页|Characterization of serum proteomic and inflammatory profiling at early stage of iron deficiency in weaned piglets

Characterization of serum proteomic and inflammatory profiling at early stage of iron deficiency in weaned piglets

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The objective of this study was to examine the early serum proteomic and inflammatory profiles of weaned piglets subjected to iron deficiency.Twelve healthy piglets(Duroc x Landrace x Large Yorkshire,body weight:4.96±0.05 kg)were weaned at 21 days of age.Subsequently,these animals were randomly allocated to one of two groups,with six replicates in each group(maintaining a male-to-female ratio of 1:1),the control group(administered 100 mg/kg Fe as FeSC4·H2O)and L-Fe group(no additional Fe supplementation).The results showed that 42 days after initiating,compared with control group,routine blood analysis revealed a reduction in serum iron content,red blood cell(RBC)count,hemoglobin(HGB)content,hematocrit(HCT),and mean corpuscular volume(MCV)(P＜0.05).Subsequent sample analysis indicated a noteworthy decrease in iron deposition in the liver,spleen,and kidneys of piglets fed the L-Fe diet compared with control group(P＜0.05).However,final body weight,average daily gain(ADG),average daily feed intake(ADFI),feed conversion ratio,and tissue coefficients were similar between the two groups(P＞0.05).During the early stages of iron deficiency,piglets exhibited increased villus height(VH)and the ratio of VH to crypt depth(CD)in the duodenum(P＜0.05)and increased expression levels of iron transporters,including duodenal cytochrome(Cybrd),divalent metal transport 1(DMT1),and ferritin light chain(FTL)(P＜0.05).Subsequently,isobaric tags for relative and absolute quantitation(iTRAQ)were used to identify serum proteins.Gene Ontology(GO)analysis of the differentially abundant proteins(DAP)revealed that 24 of the 30 DAP were involved in platelet function,immune response,cellular metabolism,transcription,and protein synthesis.Notably,prothrombin,asporin(ASPN),and Rac family small GTPase 3(RAC3)expression was induced,whereas glycoprotein Ⅰb platelet subunit alpha(GPIbA)expression was decreased.This was accompanied by a substantial reduction in serum comple-ment 3(C3)and complement 4(C4)contents(P＜0.05),with elevated the contents of interleukin-1β(IL-1β),interleukin-4(IL-4),interleukin-6(IL-6),transforming growth factor-β1(TGF-β1),and tumor ne-crosis factor-α(TNF-α)(P＜0.05).Our findings underscore the essential role of dietary iron supple-mentation in maintaining iron homeostasis and modulating inflammatory responses in piglets.

PigletIron deficiencyProteomicsInflammatoryCytokineImmune response

Guang Liu、Lan Li、Shuan Liu、Zhenglin Dong、Jian Zhou、Chengyan Gong、Yulong Yin、Wenjie Tang、Dan Wan

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Laboratory of Animal Nutritional Physiology and Metabolic Process,Key Laboratory of Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China

Hubei Hongshan Laboratory,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China

Beijing Dabeinong Technology Group Co.,Ltd.,Beijing 100080,China

University of Chinese Academy of Sciences,Beijing 101408,China

Animal Breeding and Genetics Key Laboratory of Sichuan Province,Sichuan Animal Science Academy,Chengdu 610066,China

Livestock and Poultry Biological Products Key Laboratory of Sichuan Province,Sichuan Animtech Feed Co.,Ltd.,Chengdu 610066,China

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National Key R&D Program of ChinaNational Natural Science Foundation of Chinascience and technology innovation Program of Hunan ProvinceYouth Innovation Promotion Association of Chinese Academy of Sciences

2022YFD1300504323728272023RC32012022370

2024

10.1016/j.aninu.2024.04.004

动物营养(英文)

动物营养(英文)

CSTPCD
ISSN：
年,卷(期)：2024.18(3)