Abstract
Pogostemon cablin essential oil(PEO),extracted from P.cablin,has anti-oxidant,anti-inflammatory,and anti-stress properties,as well as the ability to improve gastrointestinal digestion.This study aims to evaluate the effects of PEO on the performance,rumen epithelial morphology,and barrier function in heat-stressed beef cattle.Thirty-six male Jingjiang cattle at 18 months old were randomly assigned into four groups and fed a diet containing PEO at 0(control),50,100,or 150 mg/kg in the feed concentrate(n=9).All experimental cattle were fed under high temperature and humidity in summer for 60 days.The results indicated that 50 mg/kg of PEO treatment enhanced the average daily gain of beef cattle compared with the control group(P=0.032).All PEO treatments reduced the diamine oxidase activity(P=0.004)and malondialdehyde content(P=0.008)in serum.In addition,the content of 70 kDa heat shock protein in the 100 mg/kg group was increased,and the activity of glutathione peroxidase and total antioxidant capacity in both 100 mg/kg and 150 mg/kg groups were enhanced compared to the control group(P＜0.05).More importantly,PEO treatment with 50 mg/kg enhanced the mRNA relative ex-pressions of occludin in ruminal epithelia but decreased the mRNA relative expressions of c-Jun N-ter-minal kinase,P38 mitogen-activated protein kinases,caspase-3,Beclin1(P＜0.05),and extremely significant declined the mRNA relative expressions of extracellular regulated protein kinases and ubiquitin-binding protein in contrast to the control group(P＜0.01).These findings indicated that dietary PEO supplementation might be favorable to improve growth performance and repairing damaged rumen epithelium of heat-stressed cattle by down-regulating the mitogen-activated protein kinase signaling pathway.
基金项目
National Natural Science Foundation of China(32060768)
Central guidance for local scientific and technological development funding projects of China(20231 zdf03083)
China Agriculture Research System of MOF and MARA(CARS-37)
NETL
NSTL
万方数据