首页|Methionine supplementation regulates eggshell quality and uterine transcriptome in late-stage broiler breeders
Methionine supplementation regulates eggshell quality and uterine transcriptome in late-stage broiler breeders
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This study aimed to compare the effects of dietary methionine(Met)and 2-hydroxy-4-(methylthio)-butanoate(HMTBA)on the eggshell quality of broiler breeder hens and elucidate the mechanism of Met in improving eggshell quality from the perspectives of eggshell microstructure and shell gland physio-logical function.A total of 720 WOD188 broiler breeder hens at 40 weeks old were assigned to 3 groups,with 8 replicates per group and 30 birds per replicate.Over 7 weeks,birds were fed a basal diet or the same diet supplemented with 0.15％Met or 0.17％HMTBA.Our findings revealed significant improve-ments in the Met group for egg shape index,shell thickness,breaking strength,and fracture toughness(P＜0.05),whereas the HMTBA group showed no significant improvements(P＞0.05).Met supple-mentation increased calcium and phosphorus levels in both serum and shell gland tissue(P＜0.05),and enhanced Ca2+ATPase activity in shell gland tissue(P＜0.05).Histomorphological changes cluded enhanced mucosal fold dimensions and increased epithelial height in the shell gland(P＜0.05).Met also improved eggshell ultrastructure,resulting in a thicker effective layer and broader mammillae with fewer type B structures(P＜0.05).The mRNA levels for genes regulating eggshell ultrastructure,such as ovocleidin-116(OC-116),calbindin 1(CALB1),and integral membrane protein 2C(ITM2C),were signifi-cantly upregulated in the Met group(P＜0.05).Transcriptome analysis identified 248 differentially upregulated genes in the Met group,primarily linked to the non-canonical Wnt/Ca2+signaling pathway,crucial for calcium ion transport and cellular proliferation.This research highlights that Met supple-mentation improves eggshell quality by enhancing calcium transport and cellular proliferation in uterine function,particularly through the modulation ofWnt family member 11(WNT11)and CALB1,influencing calcium deposition and ultrastructural development.
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