Effects of Plant Essential Oil on Rumen Fermentation and Methanogenesis Studied Using a Dual Flow Continuous Culture System
This experiment was conducted to study the effects of essential oil supplemental level on rumen fermentation and methanogenesis using a dual flow continuous culture system.The four supplemental levels (0,100,500 and 1 500 mg/L) of cinnamon oil or spearmint oil were used as the treatment factors,there were 3 replicates per treatment and each experiment lasted for 6 days.The results showed as follows:compared with the control group,1) the supplementation of 1 500 mg/L cinnamon oil significantly decreased the pH,neutral detergent fibre degradation rate,acid detergent fiber degradation rate and crude protein degradation rate (P ＜0.05).2) There were no significant differences in the concentration of volatile fatty acid and acetate/propiohate ratios (A/P) in 100 mg/L cinnamon oil or spearmint oil groups (P ＞0.05).The concentrations of total volatile fatty acid (TVFA),acetate and propionate were significantly decreased in 500 mg/L cinnamon oil group at 27 and 99 h and 1 500 mg/L group at all the fermentation time (P ＜0.05).With the prolonging of fermentation time,the concentrations of TVFA,acetate and propionate were significantly decreased (P ＜0.05),and A/P was significantly increased in 1 500 mg/L cinnamon oil group (P ＜ 0.05).3) The supplementation of 1 500 mg/L cinnamon oil or 500 mg/L spearmint oil significantly decreased the number of protozoa (P ＜ 0.05).The methane volume fraction in all groups was decreased compared with the control group (P ＜ 0.05).It is concluded that the supplementation of 100 mg/L cinnamon oil or spearmint oil may be beneficial to manipulate rumen microbial fermentation.
essential oilcontinuous culturerumen fermentation in vitromethane
国家科技期刊平台
NSTL
万方数据
维普
