Effects of Unsaturated Fatty Acids on Lipid Metabolism Enzyme Activities and Muscle Components of Juvenile Coilia nasus
In order to evaluate the effects of unsaturated fatty acids (UFA) on lipid metabolism enzyme activities and muscle components of juvenile Coilia nasus,a total of 3 000 juvenile Coilia nasus with an initial body weight of (2.78 ± 0.09) g were randomly divided into two groups (three replicates per group and 500 fish per replicate),and the fish in the two groups were fed two formulated diets contained different levels of lipid.One diets contained 4.76％ fish oil (additive source of UFA) and the other diet did not contain fish oil (the measured levels of crude lipid were 11.32％ and 7.86％,respectively).Then the fish from two groups were fed the two diets for 60 days.The results showed that there were no significant differences between two groups in the final average body weight,final body length,body length/body height,body length/body thickness,hepatosomatic index (HSI) and viserosomatic index (VSI) (P ＞ 0.05),while the condition factor (CF) in adding group was significantly higher than that in non-adding group (P ＜ O.05).Compared with non-adding group,the muscle crude protein content in adding group was significantly decreased (P ＜ 0.05),while the muscle ether extract content showed an increasing trend (P ＞ 0.05).Furthermore,there were no significant differences between two groups in the activities of lipase (LPS) and total lipoidase (TLP) and lipid peroxidation (LPO) content in liver (P ＞0.05).In liver,the activities of carnitine palmityl transferase-Ⅰ (CPT-Ⅰ)and carnitine palmityl transferase-Ⅱ (CPT-Ⅱ) in adding group were significantly lower than those in nonadding group (P ＜ 0.05),while the acetyl-CoA carboxylase (ACC) activity was significantly higher than that in non-adding group (P ＜ 0.05).In muscle,the activities of CPT-Ⅰ,CPT-Ⅱ and ACC were not significantly different between two groups (P ＞ 0.05).In addition,the contents of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) in muscle were significantly decreased and the contents of polyunsaturated fatty acids (PUFA),eicosapntemacnioc acid (EPA),docosahexaenoic acid (DHA) and n-3 PUFA in muscle were significantly increased after added 4.76％ fish oil into the diet (P ＜ 0.05).In conclusion,improving UFA content in diets can effectively increase the content of essential fatty acid of juvenile Coilia nasus,which is conductive to improve nutrition value of fish,but has a trend to increase the LPO content of fish.
国家科技期刊平台
NSTL
万方数据
维普
