Analysis of Structure and Diversity of Salivary Bacteria of Healthy Pigs Using Polymerase Chain Reaction-Denaturing Gradient Gel ElectroPhoresis
This experiment was conducted to study the structure and diversity of salivary bacteria of healthy pigs. The bacterial diversity was studied using polymerase chain reaction-denaturing gradient gel electrophoresis ( PCR-DGGE),cluster analysis and principal component analysis. Some common and special bands were iden-tified by clone and sequencing at the same time. The results showed that in PCR-DGGE profile,the average band number of piglets was 23 and the average band number of finishing pigs was 28;the diversity index, richness and evenness of finishing pigs were higher,and they were 3.34,0.877 and 28.40,respectively,while the diversity index,richness and evenness of piglets were 3.17,0.834 and 23.20,respectively;the cluster a-nalysis suggested that there were some differences in salivary bacterial population during different growth stages of pigs,but the similarity coefficient in all the samples was higher than 0.66. In PCR-DGGE profile,Lactoba-cillus fermentum,Escherichia coli,Mycobacterium arosiense,Firmicutes and Streptococcus suis were predom-inant in mouth cavity of pigs;the special bacteria in saliva of piglets were Neisseria dentiae and Neisseria shayeganii,and the special bacteria in saliva of finishing pigs was Staphylococcus sp.. There is a significant difference in the diversity of salivary bacteria between piglets and finishing pigs. The diversity of salivary bacte-ria is increasing and gradually stabilized with the growth of pigs.
国家科技期刊平台
NSTL
万方数据
维普
