Effects of Nicitinic Acid, Fructooligosaccharides and Rare Earth Citrate Combinations on in Vitro Rumen Fermentation Characteristics and Bacterial Community of Beef Cattle
In order to study the effects of nicitinic acid(NA), fructooligosaccharides(FOS)and rare earth citrate(REC)combinations on in vitro rumen fermentation characteristics and bacterial community of beef cat-tle, this experiment was designed according to three factors and three levels orthogonal experiment design. Three healthy Jinjiang cattle[body weight was(375±28)kg]with permanent ruminal cannulas were adopted for rumen support animals. The effects of adding different amounts of FOS(0.8%, 1.0% and 1.2%), NA (400,800 and 1 200 mg/kg)and REC(0.6%, 0.8% and 1.0%)on rumen fermentation characteristics of beef cattle were studied by in vitro rumen fermentation culture technology,and rumen bacterial community was analyzed by high throughput sequencing technology. Nine regulator combinations were designed, which were 0.8% FOS+400 mg/kg NA+0.6% ERC(group A), 0.8% FOS+800 mg/kg NA+0.8% ERC(group B), 0.8% FOS+1 200 mg/kg NA+1.0% ERC(group C),1.0% FOS+400 mg/kg NA+0.8% ERC(group D), 1.0% FOS+800 mg/kg NA+1.0% ERC(group E),1.0% FOS+1 200 mg/kg NA+0.6% ERC(group F), 1.2% FOS+400 mg/kg NA+1.0% ERC(group G), 1.2% FOS+800 mg/kg NA+0.6% ERC(group H), 1.2% FOS+1 200 mg/kg NA+0.8% ERC(group I), respectively. Each regulator combination had 3 culture bottles. The in vitro culture time was 48 hours. The results showed as follows:1)the pH of in vitro cultivated fluid was highest in group G,it was 6.77,which was significantly higher than that in groups A,B,C,D and E(P≤0.05); the ammoniacal nitrogen(NH3-N)content of in vitro cultivated fluid was lowest in group G,it was 9.77 mg/dL,which was significantly lower than that in other groups(P≤0.05);no significant difference was found in microbial protein(MCP)content of in vitro cultivated fluid among groups(P>0.05). 2)The propionic acid content of in vitro cultivated fluid was highest in group I,it is 28.53%,which was significantly higher than that in groups A,B,D and E(P≤0.05);the acetic acid/propionic acid value of in vitro cultivat-ed fluid was lowest in group I,which was significantly lower than that in groups A,B and D(P≤0.05);no significant difference was found in the total volatile fatty acid(TVFA)concentration, the contents of acetic acid and butyrate acid of in vitro cultivated fluid among groups(P>0.05). 3)The dominant bacterial commu-nities of different regulator combinations all were Bacteroidete and Firmicultes. At phylum level, there were 4 bacterial communities had significant differences between groups A and I(P≤0.05); at genus level, there were 15 bacterial communities had significant differences between groups A and I(P≤0.05). The results indi-cate that the better rumen regulator combinations for corn-soybean meal-rice straw type diets of beef cattle are 1.2% FOS+1 200 mg/kg NA+0.8% ERC and 1.2% FOS+400 mg/kg NA+1.0% REC.
beef cattlefructooligosaccharidenicotinic acidrare earth citraterumenin vitro fermentationbacterial community
国家科技期刊平台
NSTL
万方数据
维普
