This experiment was conducted to explore the effects of three probiotics combined with acidifiers on growth performance,antioxidant capacity and intestinal health of broilers,aiming to provide evidence for the combination of probiotics and acidifiers.A total of 875 male Arbor Acres broilers of 1-day-old were randomly divided into 5 groups as control group(CON group),acidifier group(ACI group),acidifier+Bacillus licheni-formis group(ACI+BL group),acidifier+Bacillus coagulans group(ACI+BC group)and acidifier+Clostridi-um butyricum group(ACI+CB group)with 7 replicates per group and 25 broilers per replicate.Broilers in CON group were fed a basal diet,those in ACI group were fed a basal diet supplemented with 250 mg/kg a-cidifier,and those in ACI+BL group,ACI+BC group and ACI+CB group were fed the basal diet supplemen-ted with 250 mg/kg acidifier as well as Bacillus licheniformis(1 to 21 days of age,9xl09 CFU/kg;22 to 42 days of age,3xl09 CFU/kg),Bacillus coagulans(1 to 21 days of age,3.0×1010 CFU/kg;22 to 42 days of age,l.0×1010 CFU/kg)and Clostridium butyricum(1 to 21 days of age,1.5×109 CFU/kg;22 to 42 days of age,0.5×109 CFU/kg),respectively.The experiment lasted for 42 days.The results showed as follows:1)compared with CON group,the average daily gain(ADG)of broilers from 1 to 21 days of age,the villus height to crypt depth ratio(V/C)in jejunum at 21 days of age,and the lipid activity in jejunum and the zonu-la occludens-1(ZO-1)mRNA relative expression level in jejunal mucosa at 42 days of age in ACI group were significantly increased(P＜0.05);the bursa of Fabricius index at 21 days of age,the serum catalase(CAT)activity at 42 days of age,the villus height(VH)in ileum at 21 days of age,the V/C in jejunum and ileum at 21 and 42 days of age,and the activities lipase and trypsin in jejunum and ZO-1 mRNA relative expression lev-el in jejunal mucosa at 42 days of age in ACI+BL group were significantly increased(P＜0.05);the ADG from 1 to 21 days of age,the bursa of Fabricius index and glutathione peroxidase(GPX)activity in serum at 21 days of age,the activities of CAT and superoxide dismutase(SOD)in serum at 42 days of age,the VH in ileum at 21 days of age,the V/C in jejunum and ileum at 21 and 42 days of age,and the activities of lipase and trypsin in jejunum and mRNA relative expression levels of ZO-1,nuclear factor E2-related factor 2(Nrf2),SOD and interleukin-10(IL-10)in jejunal mucosa at 21 and 42 days of age in ACI+BC group were significantly increased(P＜0.05),while the ratio of feed to gain(F/G)in each growth stage and the malondi-aldehyde(MDA)content in serum as well as the mRNA relative expression levels of nuclear factor-KB(NF-κB)and tumor necrosis factor-α(TNF-α)in jejunal mucosa at 42 days of age were significantly de-creased(P＜0.05);the ADG from 1 to 21 days of age,the activities of CAT and SOD in serum at 42 days of age,the V/C in jejunum and ileum at 21 and 42 days of age,and the relative mRNA expression levels of ZO-1 and Nrf2 in jejunum mucosa at 42 days of age in ACI+CB group were significantly increased(P＜0.05),while the F/G in each growth stage and the TNF-α mRNA relative expression level in jejunum mucosa at 42 days of age were significantly decreased(P＜0.05).2)Compared with ACI group,the bursa of Fabricius index and GPX activity in serum of broilers at 21 days of age,the activities of CAT and SOD in serum at 42 days of age,the VH in ileum at 21 days of age,the V/C in ileum at 42 days of age,and the trypsin activity in jejunum and mRNA relative expression levels of ZO-1,SOD and IL-10 in jejunum mucosa at 42 days of age in ACI+BC group were significantly increased(P＜0.05),while the MDA content in serum and NF-κB mRNA relative ex-pression level in jejunum mucosa at 42 days of age were significantly decreased(P＜0.05);the activities of CAT and SOD in serum and the V/C in jejunum and ileum at 42 days of age in ACI+CB group were signifi-cantly increased(P＜0.05).In conclusion,the combination of probiotics and acidifiers can improve growth performance,immunity,antioxidant capacity and intestinal health of broilers,and the effect is better than that of acidifier alone.The mechanism of combining probiotics with acidifiers to improve intestinal health may be related to promoting the Nrf2 antioxidant pathway and inhibiting the expression of NF-κB inflammatory path-way related factors.Under the experimental conditions,the combination of Bacillus coagulans and acidifier has a better effect.[Chinese Journal of Animal Nutrition,2023,35(12):7759-7773]
broilersprobioticsacidifiersgrowth performanceantioxidant capacityintestinal health
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