The purpose of this experiment was to explore the effects of β-sitosterol(BSS)on oxidative stress and milk fat synthesis of mammary epithelial cells(HC11 cells)induced by lipopolysaccharide(LPS).The HC11 cells cultured in vitro were used as a model,and divided into 5 groups:control group(CON group,growth medium treated),mammary inflammatory model group(LPS group,1 μg/mL LPS treated)and in-flammatory model medicine groups(BSS+LPS groups,after 5,10 and 20 μmol/L BSS treated 1 h,respec-tively,added 1 μg/mL LPS),and set 5 replicates per group.The oxidative stress indexes and the mRNA ex-pression and protein expression of antioxidant related genes and milk fat synthesis related genes in HC11 cells were detected.The results showed as follows:1)compared with the CON group,the malondialdehyde(MDA),nitric oxide(NO)contents and inducible nitric oxide synthase(iNOS)activity in HC11 cells of LPS group were significantly increased(P＜0.05),and the superoxide dismutase(SOD),glutathione peroxi-dase(GPx),catalase(CAT)activities and total antioxidant capacity(T-AOC)were significantly decreased(P＜0.05).Compared with the LPS group,the MDA,NO content and iNOS activity in HC11 cells of 5,10 and 20 µmol/L BSS+LPS groups were significantly decreased(P＜0.05),and the SOD,GPx,CAT activities and T-AOC were significantly increased(P＜0.05).2)Compared with the CON group,the mRNA and pro-tein relative expression levels of nuclear factor erythroid 2-related factor 2(Nrf2),heme oxygenase-1(HO-1)and NAD(P)H:quinone oxidoreductase 1(NQO1)in HC11 cells of LPS group were significantly decreased(P＜0.05).Compared with the LPS group,the mRNA and protein relative expression levels of Nrf2,HO-1 and NQO1 in HC11 cells of 5,10 and 20 µmol/L BSS+LPS groups were significantly increased(P＜0.05).3)Compared with the CON group,the mRNA and protein relative expression levels of peroxisome proliferator activated receptor y(PPARy)and sterol regulatory element binding protein 1(SREBPl)in HC11 cells of LPS group were significantly decreased(P＜0.05),and the mRNA relative expression levels of acetyl-CoA carboxylase(ACC),fatty acid synthase(FAS)and stearyl coenzyme A desaturated enzyme(SCD)were sig-nificantly decreased(P＜0.05).Compared with the LPS group,the mRNA and protein relative expression lev-els of PPARy and SREBP1 in HC11 cells of 5,10 and 20 µmol/L BSS+LPS groups were significantly in-creased(P＜0.05),and the mRNA relative expression levels of ACC,FAS and SCD were significantly in-creased(P＜0.05).In conclusion,BSS can inhibit LPS-induced HC11 cells oxidative damage,and promote milkfat synthesis in HC11 cells under oxidative stress.[Chinese Journal of Animal Nutrition,2023,35(12):8013-8023]
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