The aim of this study was to explore the mechanism of water-soluble active components of Moringa oleifera leaves in treating enteritis by using network pharmacology and molecular docking technology.The wa-ter-soluble active components of Moringa oleifera leaves were collected through literature search,the chemical structure was obtained in PubChem database,the potential targets were predicted by Swiss Target Prediction da-tabase,and the overlapping targets of the active components of Moringa oleifera leaves and enteritis were ob-tained by GeneCards database.Subsequently,the above overlapping targets were imported into STRING data-base to construct protein-protein interaction(PPI)network,and core targets were screened by Cytoscape soft-ware CytoNCA plug-in.Then DAVID database was used to conduct gene ontology(GO)functional enrich-ment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathway enrichment analysis for the selected core targets,and the R clusterProfiler package was used to analyze the enrichment targets in key pathways.Finally,the core targets and the active components were docked by Autodock-Tools software.The results showed as follows:1)a total of 31 water-soluble active components of Moringa oleifera leaves were se-lected,including sinapic acid,cinnamic acid,kaempferol,quercetin,naringin,moringa,niazimin A and pro-tocatechuic acid,and there were 35 corresponding core targets.2)PPI network analysis showed that SRC pro-to-oncogene,non-receptor tyrosine kinase(SRC),epidermal growth factor receptor(EGFR),matrix metal-loproteinase 9(MMP9),protein kinase B1(AKT1),prostaglandin endoperoxide synthase 2(PTGS2)and heat shock protein 90 alpha family class A member 1(HSP90AAl)were the key targets of Moringa oleifera leaves in treating enteritis.3)Enrichment analysis of KEGG signaling pathway showed that the selected core targets were mainly involved in phosphatidylinositol 3-kinase(PI3K)-protein kinase B(AKT),Toll-like re-ceptor(TLR),tumor necrosis factor(TNF),mitogen-activated protein kinase(MAPK),NOD-like receptor and other signaling pathways,and EGFR,MMP9,RELA proto-oncogene nuclear factor-KB subunit(RE-LA),SRC,AKT1,nuclear factor-κB subunit 1(NFKB1),mitogen-activated protein kinase 8(MAPK8),Toll-like receptor 4(TLR4),HSP90AA1,signal transducer and activator of transcription 3(STAT3),vascu-lar endothelial growth factor A(VEGFA)and CREB binding protein(CREBBP)had been used to treat enter-itis.4)Molecular docking prediction results showed that sinapic acid and niazimin A could stably bind to key targets.In conclusion,the active components of Moringa oleifera leaves can play anti-inflammatory and antivi-ral effects through corresponding signaling pathways,and can also regulate biological processes such as cell dif-ferentiation and apoptosis,which provides a theoretical basis for the further development and application of Moringa oleifera leaves in intestinal diseases.[Chinese Journal of Animal Nutrition,2023,35(12):8053-8073]
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