The aim of this experiment was to investigate whether the oral administration of Broussonetia papy-rifera leaf polysaccharides(BPP)could alleviate the negative effects of the diet containing oxidized fish oil on liver health in rats,and to primarily explore the possible mechanisms.Thirty-two male Wistar rats weaned at 21 days of age(approximate average weight was 58.05 g)were used in a 2x2 factorial design.The trial factors included oral administration of BPP(oral administration of BPP solution or phosphate buffered solution)and oxidized fish oil challenge(feeding the diets containing oxidized fish oil or normal fish oil).The daily oral dose of BPP was 22.5 mg/kg BW.From day 9 to day 26,the challenge of oxidized fish oil was executed.The trial duration was 26 days.The results showed that oxidized fish oil challenge increased diarrhea rate,signifi-cantly enhanced glutathione transaminase and glutamic acid transaminase activities in liver and serum and urea nitrogen content in serum of rats(P＜0.05),significantly upregulated the mRNA relative expression levels of cysteine aspartate protease 3 and Kelch-like epichlorohydrin-associated protein-1 and the ratio of B lymphocyto-ma-2-associated X protein to B lymphocytoma-2 in liver of rats(P＜0.05),significantly down-regulated the mRNA relative expression levels of B lymphocytoma-2 in liver of rats(P＜0.05),significantly decreased cata-lase and superoxide dismutase activities and total antioxidant capacity in serum of rats(P＜0.05),significantly reduced catalase and glutathione peroxidase activities and total antioxidant capacity in liver of rats(P＜0.05),significantly increased malondialdehyde,lipid peroxides and protein carbonyls contents in serum of rats(P＜0.05),and significantly enhanced malondialdehyde content in liver of rats(P＜0.05).Oral administration of BPP reduced diarrhea rate of rats fed the diet containing oxidized fish oil,and significantly alleviated the nega-tive effects of oxidized fish oil challenge on liver-function-associated indices and antioxidant-related indices in serum and liver,and the expression of apoptosis-related genes and antioxidant-genes in liver of rats(P＜0.05).In summary,oral administration of BPP can alleviate the diarrhea and liver damage induced by oxidized fish oil challenge in rats,which may be related to the improvement of antioxidant function and apoptosis through regu-lating nuclear factor erythroid-2(Nrf2)signaling pathway.[Chinese Journal of Animal Nutrition,2023,35(12):8074-8082]
Broussonetia papyrifera leaf polysaccharidesoxidized fish oilratsoxidative stressliveran-tioxidant capacity
国家科技期刊平台
NSTL
万方数据
