In order to explore the effects of kaempferitrin from Siraitia grosvenorii on the antioxidant capacity of chronic sleep deprived mice,fifty-six 4-week-old male specific pathogen free(SPF)ICR mice were used as subjects and fed adaptively for one week.According to their body weight,they were randomly divided into blank control group,model control group,positive control group,dimethyl sulfoxide(DMSO)control group,low-kaempferitrin group,medium-kaempferitrin group and high-kaempferitrin group,there were 8 rats in each group,4 replicates in each group and 2 replicates in each group.There was no significant difference in body weight among the groups(P＞0.05).The rats in other experimental groups except the blank control group were given chronic sleep deprivation for 4 weeks and were given intragastric administration for 4 weeks after success-ful modeling.The blank control group and the model control group were given 0.5 mL saline per day,the posi-tive control group was given 30 mg/kg BW melatonin per day,the DMSO control group was given 0.5 mL 1％DMSO solution per day,and the low-,medium-and high-kaempferitrin groups were given 15,30 and 60 mg/kg BW kaempferitrin from Siraitia grosvenorii per day,respectively.After the gavage,the body weight,carcass weight,heart weight,liver weight and body fat content of mice were.determined,the activities of superoxide dismutase(SOD),glutathione peroxidase(GPX),heme oxygenase-1(HO-1),quinone oxi-doreductase 1(NQO1),total antioxidant capacity(T-AOC)and malondialdehyde(MDA)content in heart and liver were determined,and the mRNA relative expression levels of antioxidant-related genes such as total transcription factor NF-E2 related factor 2(Nrf2),SOD-1,SOD-2,GPX-1,GPX-4,HO-1 and NQO1 in heart and liver were measured.The results showed that administration of 15,30 or 60 mg/kg BW kaempferitrin from Siraitia grosvenorii per day could significantly reduce the body weight and body fat rate of chronic sleep deprived mice(P＜0.05),and significantly improve the indexes of heart and liver of chronic sleep deprived mice compared with the model control group(P＜0.05).The activities of SOD,GPX,HO-1 and NQO1 in heart of chronic sleep deprived mice in the low-,medium-and high-kaempferitrin groups were significantly higher than those in the model control group and DMSO control group(P＜0.05),and the MDA content was significantly lower than that in the model control group and DMSO control group(P＜0.05).The T-AOC in heart of chronic sleep deprived mice in the medium-and high-kaempferitrin groups was significantly higher than that in the model control group and DMSO control group(P＜0.05).The activities of SOD,GPX,HO-1,NQO1 and T-AOC in liver of chronic sleep deprived mice in the medium-and high-kaempferitrin groups were significantly higher than those in the model control group and DMSO control group(P＜0.05),and the MDA content was significantly lower than that in the model control group and DMSO control group(P＜0.05).The relative expression levels of SOD-1,SOD-2,GPX-1,GPX-4,Nrf2,HO-1 and NQO1 mRNA in heart and liver of chronic sleep deprived mice in the medium-and high-kaempferitrin groups were significant-ly higher than those in the model control group and DMSO model control group(P＜0.05).In conclusion,kaempferitrin from Siraitia grosvenorii can significantly increase the activities of antioxidant enzymes and the expression of antioxidant-related genes in heart and liver of chronic sleep deprived mice,and then improve the oxidative stress injury of chronic sleep deprived mice and enhance their antioxidant capacity.[Chinese Journal of Animal Nutrition,2023,35(12):8083-8096]
kaempferitrin from Siraitia grosvenoriimicechronic sleep deprivationantioxidant capacity
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