The aim of this study was to evaluate the probiotic properties and safety of canine-derived Pediococ-cus acidilactici GLP236.The taxonomic status of the strain was identified by Gram staining,16S rRNA se-quencing and physiological and biochemical characteristics.The probiotic properties and safety of the strain were evaluated by low pH,bile salts,gastrointestinal environment and high-temperature tolerance,strain self-coagulation,surface hydrophobicity,antioxidant activity,hemolytic activity,bacteriostatic activity,antibiotic susceptibility and mice instillation test.In the mice instillation test,eighty healthy Kunming mice with similar body weight were randomly divided into 4 groups with 20 mice in each group.Mice in the control group were orally administered 0.2 mL sterile physiological saline,and mice in other groups were orally administered 0.2 mL Pediococcus acidilactici GLP236 with concentrations of 1×109(LG group),1×1010(MG group)and 1×1011 CFU/mL(HG group),respectively.The experiment lasted for 28 days.The results showed as fol-lows:1)GLP236 was identified as Pediococcus acidilactici in combination with 16S rRNA and physiological and biochemical results.2)The strain entered the stabilization phase after 24 h of growth,and the pH was fi-nally stabilized at 4.10.3)The strain survival rates were 96.91％,86.24％and 65.73％in 0.1％,0.3％and 0.5％bile salts,respectively,and the survival rates were 73.81％,94.11％and 64.18％in artificial gastric flu-id,artificial intestinal fluid and artificial gastrointestinal fluid,respectively,and 80,90 ℃ can withstand 5 min.4)The strain self-coagulation rates were 57.02％,77.56％and 87.22％at 8,16 and 24 h,respective-ly,the surface hydrophobicity was 62.56％,and thefree radical scavenging rates of 2,2-diazo-di(3-ethyl-benzothiazole-6-sulfonic acid)diamine salt(ABTS)and 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)were 87.28％and 80.70％.5)The bacterial suspensions and cell-free cell supernatant could inhibit the growth of pathogenic bacteria,and the strains were insensitive to antibiotics such as benzoxacillin,ampicillin,gen-tamicinand so on,and showed gamma hemolysis.6)The average daily gain of MG group was significantly higher than that of CK group and LG group(P＜0.05),the average daily feed intake of MG group and HG group was significantly higher than that of CK group(P＜0.05).The serum superoxide dismutase activity of MG group and HG group was significantly higher than that of CK group(P＜0.05),and the serum malondial-dehyde content was significantly lower than that of CK group(P＜0.05);the serum aspartate aminotransferase activity of LG group,MG group and HG group was significantly lower than that of CK group(P＜0.05),the serum urea nitrogen content of MG group was significantly lower than that of CK group(P＜0.05).In conclu-sion,the canine-derived Pediococcus acidilactici GLP236 has good probiotic properties and can be used as a candidate for canine-derived probiotics.[Chinese Journal of Animal Nutrition,2023,35(12):8148-8160]
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