Effects of Dietary 5-Aminolevulinic Acid Supplementation on Growth Performance,Blood Routine Indices,Immune Function and Intestinal Morphology in Broilers
This study aimed to investigate the effects of dietary supplementation with 5-aminolevulinic acid(5-ALA)on growth performance,blood routine indices,immune function and intestinal morphology in broil-ers.A total of 300 one-day-old Arbor Acres broilers were distributed into 5 groups with 6 replicates of 10 broil-ers by a completely randomized design.The broilers in the 5 groups were fed the basal diet with 0(control),15,30,45,and 60 mg/kg 5-ALA,respectively.The results showed as follows:1)dietary 5-ALA supple-mentation had no significant effects on average daily feed intake(ADFI)and average daily gain(ADG).Di-etary supplementation with 60 mg/kg 5-ALA reduced the feed to gain ratio(F/G)during 1 to 21 days of age and 22 to 42 days of age(P＜0.05).Moreover,with the dietary 5-ALA supplemental level increased,the F/G was linearly decreased(P＜0.05).2)Dietary supplementation with 30,45 and 60 mg/kg 5-ALA increased the blood hemoglobin(HGB)content of broilers on 21 days of age.At the same time,with the increase of 5-ALA supplemental level,the HGB content and red blood cell counts(RBC)were linearly increased(P＜0.05).3)Dietary supplementation with 45 mg/kg 5-ALA increased the contents of immunoglobulin M(IgM)and im-munoglobulin G(IgG)in serum of broilers on 21 days of age(P＜0.05),and dietary supplementation with 30 or 45 mg/kg 5-ALA increased the contents of IgM,IgG and immunoglobulin A(IgA)in serum of broilers on 42 days of age(P＜0.05).Moreover,dietary supplementation with 45 and 60 mg/kg 5-ALA improved the contents of complement 3(C3),complement 4(C4),and interferon-γ(INF-γ)in serum of broilers on 21 days of age.And the C3 and C4 contents in serum of broilers on 21 days of age were linearly increased(P＜0.05).4)Compared with the control group,the mRNA relative expression levels of Avian(3-defensin(AvBD)1,AvBD2,AvBD7 and Cathelicidin(CATH)1,CATH2,CATH3 in liver were upregulated in 5-ALA supplementation group(P＜0.05).5)Dietary supplementation with 30 and 60 mg/kg 5-ALA increased the ileal villus height/crypt depth ratio(V/C)of broilers on 42 days of age.Moreover,with the dietary 5-ALA supplemental level increased,the villous height of duodenum was linearly increased(P＜0.05).In con-clusion,dietary supplementation with 5-ALA promotes the formation of hemoglobin,improves the growth per-formance,immune function and intestinal mucosal morphology of broilers,and the appropriate supplemental level is 45 to 60 mg/kg.[Chinese Journal of Animal Nutrition,2024,36(1):224-234]
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维普
