This study aimed to investigate the effects of soya saponin(SS)on immune function of laying hens,and to explore its deposition effect in eggs.A total of 270 twenty-one-week-old Hy-Line gray laying hens were randomly divided into 3 groups with 6 replicates in each group and 15 hens in each replicate.Birds in the control group(CTR group)were fed a basal diet,and others in 50 SS and 500 SS groups were fed the basal diets supplemented with 50 and 500 mg/kg SS,respectively.The trial lasted for 10 weeks.The results showed as follows:1)the serum β-defensin 1(β-DF1)content of 50 SS group was significantly higher than that of CTR group(P＜0.05).2)The gene expression levels of forkhead box protein 1(FOXO1)and interleukin-10(IL-10)in hypothalamus of 50 SS and 500 SS groups were significantly higher than those of CTR group(P＜0.05),and the gene expression levels of FOXO1,forkhead box protein 3(FOXO3)and IL-10 in spleen of 500 SS group were significantly higher than those of CTR group(P＜0.05).3)The blood hemoglobin(HGB)content of 500 SS group was significantly higher than that of CTR group(P＜0.05),and the blood mean corpuscular hemoglobin concentration(MCHC)of 50 SS group was significantly higher than that of CTR group(P＜0.05).4)The contents of soya saponin Ⅰ,soya saponin V,soya sapogenol A and soya sa-pogenol B in eggs of 500 SS group were significantly higher than those of CTR group(P＜0.05).In conclu-sion,dietary supplemented with 50 and 500 mg/kg SS can improve the immune function of laying hens.In ad-dition,dietary supplemented with 500 mg/kg SS can cause SS to deposit into eggs,and SS can be converted into soyasapogenol and deposited into eggs.[Chinese Journal of Animal Nutrition,2024,36(1):245-254]
维普
万方数据